Friday, April 30, 2021

New Music Friday: The Top 7 Albums Out On April 30 : All Songs Considered : NPR

Marie Ulven, who writes and records as girl in red. Her debut full-length, If I Could Make It Go Quiet , is on our shortlist of the best albums out on April 30. Jonathan Kise/Courtesy of the artist hide caption

Marie Ulven, the singer who writes and records as girl in red, has spent the past couple of years racking up more than a billion streams on the strength of her guitar-powered-pop singles, including "We Fell In Love In October," an anthem to queer romance and liberation. She's just dropped her much-anticipated, full-length debut, If I Could Make It Go Quiet , delivering a collection of songs that are both exuberant and introspective, as she sings about youthful hopes, anxieties and the uncertainty of how to make it in the world. We open this week's show with the song "Serotonin" and talk about how and why girl in red's reflections are connecting with a generation.

Also on the program: We've got the long-awaited Flying Lotus soundtrack to the Netflix series Yasuke , a collaboration between Matt Sweeney and Bonnie "Prince" Billy that comes more than 15 years after their previous work together and more. WNXP 's Jewly Hight joins WXPN 's John Morrison, Radio Milwaukee 's Tarik Moody, NPR Music's Alex Ramos and Stephen Thompson, along with host Robin Hilton, as they share their picks for the best albums out on April 30.

Other notable releases for April 30 : Amy Speace & The Orphan Brigade — There Used To Be Horses ; Gojira — Fortitude ; Julia Stone — Sixty Summers ; Manchester Orchestra — The Million Masks of God ; Marianne Faithful & Warren Ellis — She Walks In Beauty ; Rosie Tucker — Sucker Supreme ; Teenage Fanclub — Endless Arcade ; Tōth — You And Me And Everything.

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From Publisher: NPR.org



How Rapchat Is Disrupting Music Creation And Careers

Rapchat music-making app today announced a new $2.3 million funding round from Sony Music ... [+] Entertainment and Adjacent venture capital firm. PHOTO COURTESY OF RAPCHAT

Despite fierce competition from the likes of Facebook and TikTok, Rapchat today announced a new $2.3 million funding round from Sony Music Entertainment and Adjacent venture capital firm. Combined with the startup's original $1.7 million in seed funding, a total of $4 million in venture capital is now supporting artists working to launch careers in music. 

"We've always wanted to be the easiest way to make music on your phone, and it's evolved into aspiring artists making full-length songs on their phones," Miller says. "It all goes to show how much the markets have evolved … with the rise of the creator economy and different trends now people see the potential for music to be disrupted."

Based on a recent Rapchat survey of active users, 57% are aspiring music creators who want to make music a career or side hustle by gaining followers and monetizing their work. A total of 26% consider music a hobby and access the app to improve their music creation skills and 18% are casual music creators seeking entertainment. Exactly half of active users are international based in 160 countries. 

Miller explains that Rapchat users create original songs by accessing a catalog of more than 100,000 beats, recording vocals over beats and applying editing tools like voice effects and mastering. Creators are then able to share their work with the Rapchat community or privately with another artist. Publicly, users can share songs across major social media platforms including Instagram and TikTok. They can also use Rapchat's feedback forums, collaborations and competitions to spur engagement and grow a following. 

CULVER CITY, CA - SEPTEMBER 29: General views of Sony Music and Sony/ATV Music Publishing on the ... [+] Sony Pictures Entertainment movie studio lot on September 29, 2020 in Culver City, California. (Photo by AaronP/Bauer-Griffin/GC Images)

Nico Wittenborn, lead Investor at Adjacent, said in a statement, "(Rapchat) decreases the friction of creativity by allowing anyone, anywhere in the world to record and publish music straight from their phones. This mobile-enabled democratization of technology is what Adjacent is all about, and I am super excited to support the team in building out this next-level music platform." 

While record labels are traditionally thought of as gate keepers of the music industry, Miller says that corporate VC and innovation inside of labels has evolved greatly, making them ideal strategic investors. He points out complementary business opportunities that exist for labels such as signing and promoting new artists. 

"Sony saw the vision, they saw the future of music and what it could be with us creating our own economy," Miller says. "We're unlocking and creating a new market for those who deserve it–and bringing transparency to the process." 

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From Publisher: Forbes



For Music-Rights Deals, the Hits Keep Coming - WSJ

The flood of money into deals for rights to the song catalogs of superstar musicians isn't letting up.

Tempo Music Investments, a private-equity-backed investment firm launched in 2019 in partnership with Warner Music Group Corp. , has quietly bought up music rights from the Jonas Brothers, Florida Georgia Line, Wiz Khalifa and some top producers.

And they're not done: The firm has more than $1 billion available to keep buying, according to people familiar with the matter, an indication that the hot market for music intellectual property will continue.

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From Publisher: WSJ



Millikin launches new Music Business degree program | Millikin University

The Millikin University School of Music is pleased to announce new undergraduate degree offerings for a Bachelor of Science and a Bachelor of Arts in Music Business with a planned offering in fall 2021. Students are automatically admitted into the program upon admittance to the University; an audition is not required for admission.

Millikin's Music Business degree program will prepare students to facilitate and serve users, consumers, and artists through symbiotic relationships within the music industry. This program of study encompasses training in music fundamentals, alongside music business and traditional business coursework, designed to provide the creative practitioner the tools for a career in today's music industry.

Additionally, the Millikin School of Music is offering a full-tuition Foley School of Music scholarship. The endowed scholarship was established in 2004 by Margaret Foley, a dedicated Millikin supporter and friend. Margaret and her husband, Alden, both enjoyed attending Millikin events, especially those in the School of Music, the Millikin-Decatur Symphony Orchestra, and Kirkland Fine Arts Center performances. After Alden's passing, Margaret carried on their wishes to more fully support Millikin students by establishing this scholarship. The scholarship will be awarded to a student with a demonstrated financial need who is majoring in music business.

"Millikin has a philosophy of 'Performance Learning' that I translate as 'hands-on' and 'real world.' In many ways, some of the leaps of faith we make in the music business in trying to inspire people can be considered a performance," said Martin Atkins, coordinator of music business at Millikin. "When you are involved in a project that some people cannot even see how to start much less accomplish — that's a kind of poetry. When you brush aside doubts and push your point of view or your artist or a song — that's performance too."

The new degree program will include coursework focused on traditional business, entrepreneurial, and do-it-yourself (DIY) strategies for music performance, as well as marketing, distribution, contracts, revenue streams, merchandising, publishing, and touring. The curriculum includes hands-on learning experiences with running a record label, creating and organizing Millikin's Midwest Music Expo (MMX) with an educational/panel component, and engaging in Atkins' Tour Smart Bus Experience — a 24/7 internship immersion into the world of a real tour.

"We are adding experiences at Invisible Records Studio as well as at a music festival in Chicago during Thanksgiving, and involvement in the rollout of The Museum of Post Punk and Industrial Music in Chicago," Atkins noted.

The Music Business curriculum requires three separate internships to be completed during the program. "There are what I have been calling 'pillars of the program,'" Atkins said. "The student-run label (First Step Records), the MMX conference, the Thanksgiving concert series we are planning in Chicago, and Tour Smart Bus Edition. We also have partnerships with Tracklib — a Swedish-based sample library company that we service with music files while creating opportunities for students to immerse themselves in the realm of audio delivery and rights management. My own record label will also provide other opportunities for students to get involved."

Geared toward understanding the business tools for the 21st century musician, the degree program will assist students in finding themselves and their passions while building the mindset and the toolset to make their futures happen.

Atkins added, "You are also prepared to be a one-man army and further your own career with all of the above skills — many careers start in a 'do-it-yourself' way."

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From Publisher: Millikin University



Bay Area nonprofit Blue Bear School of Music finds creative solution to keep beat going during
From Publisher: ABC7 San Francisco



Nick Jonas to Host 2021 Billboard Music Awards | Billboard

Jonas and Clarkson are two of five musical artists who have served as sole host of the BBMAs. The others are Phil Collins , LL Cool J and Ludacris .

This is Jonas' first time as host of the Billboard Music Awards, which is two years older than he is. (He's 28; the show launched 30 years ago). But he's had lots of awards show experience, including teaming with his wife, Priyanka Chopra Jonas, to announce this year's Oscar nominations.

Jonas Brothers won three Billboard Music Awards last year: top duo/group, top radio songs artist and top radio song for "Sucker," their first No. 1 hit on the Billboard Hot 100. They were also nominated for top artist.

Jonas Brothers had great success as a teen pop act from 2007-10, landing a pair of No. 1 albums on the Billboard 200 and a Grammy nomination for best new artist. Remarkably, they have come back even stronger in the past two years with a third No. 1 album on the Billboard 200 and another Grammy nomination, best pop duo/group performance for "Sucker."

Jonas has also had success as a solo artist, notably with "Jealous," a top 10 hit on the Hot 100. Jonas teamed with Nick Monson and Justin Tranter to co-write the Golden Globe-nominated song "Home" for the 2017 movie Ferdinand . Also as a solo artist, Jonas received the Hal David Starlight Award from the Songwriters Hall of Fame in 2016.

As an actor, Jonas has appeared in such films as Goat , Jumanji: Welcome to the Jungle , Midway and Chaos Walking .

Jonas' selection as host was announced Friday (April 30), one day after this year's BBMA finalists were revealed. The Weeknd is this year's leading nominee with 16 nods, followed by Da Baby (11), Pop Smoke (10) and Gabby Barrett (nine).

This year's awards are based on the chart period of March 21, 2020, through April 3, 2021. Billboard Music Awards finalists and winners are based on key fan interactions with music, including album and digital song sales, streaming, radio airplay, and social engagement, tracked by Billboard and its data partners, including MRC Data.

The 2021 Billboard Music Awards is produced by dick clark productions. Barry Adelman and Robert Deaton are executive producers.

From Publisher: Billboard



European Authorities Accuse Apple of Distorting Music Streaming Market | Hollywood Reporter

The announcement of the new case comes just days before a high-profile trial commences in Oakland, California, between Epic Games and Apple. Epic is suing Apple after trying to evade the 30 percent "tax" for purchases within Fortnite . After Epic bypassed Apple's direct payment system, Apple pulled Fortnite from its App Store. What's resulted is a closely watched case that may impact the streaming's future and feature top executives from both companies along with those at Microsoft, Alphabet and elsewhere.

The pursuit of charges also occurs as European lawmakers consider a Digital Markets Act that would enact new rules on so-called gatekeepers, including restrictions on how platforms achieve preferential treatment for their own goods.

From Publisher: The Hollywood Reporter



Tencent Music Targeted by China Regulator | Billboard

Jay Chou performs during a launch ceremony of OPPO R15 on March 31, 2018 in Shenzhen, Guangdong Province of China.

Chinese authorities are preparing to slap down Tencent Music Entertainment and its parent company, Tencent Holdings, for anti-competitive behavior, according to media reports. Those actions threaten to force the music giant to unload streaming apps Kuwo and Kugou, shed more exclusive artist and label deals and pay a hefty fine.

Tencent Holdings should expect to pay a penalty of at least 10 billion yuan ($1.54 billion) as part of a sweeping anti-trust clampdown by the State Administration of Market Regulation (SAMR), China's competition regulator, Reuters reports, citing two people with direct knowledge of the matter.

The regulatory action could result in Tencent's music arm, which has grown into a powerhouse since being spun off and listed on the New York Stock Exchange in 2018, becoming a smaller company once the dust settles. The Chinese regulator is looking to loosen the company's stranglehold on the streaming market, which has tightened since Tencent Music acquired competitors Kuwo Music and Kugou Music in 2016.

Those acquisitions helped Tencent to pursue exclusive streaming rights with the major record labels, which have been eager to push deeper into China's burgeoning streaming market. Streaming revenue in China, the world's seventh largest music market, grew by 34% to $718.1 million in 2020, according to the IFPI.

Reports of looming regulatory action in China sent shares of Tencent Music (TME) down 5.4% to $17.80 on Thursday. The stock has fallen almost 17% over the past month.

The regulatory crackdown is part of a broader push by the government of China's president Xi Jinping to clip the wings of the country's internets giants, which grew rapidly under looser regulation earlier in his administration, which began in 2013. Under Jinping and former president Hu Jintao , the Chinese government adopted a somewhat hands-off approach toward the nascent e-commerce, internet and digital-finance areas of the economy.

But lately Xi Jinping's government has been looking to limit the economic and social influence of China's most powerful corporations and their billionaire founders like Jack Ma , founder of Ant Group Company.

Companies that concern the Communist Party include Tencent and Alibaba Group Holding, private internet firms that collect valuable data from hundreds of millions of consumers which the party can't easily control. Tencent, Ant and Alibaba had a combined market capitalization of nearly $2 trillion in early November, according to Bloomberg , a sum that surpassed state-owned Bank of China as the country's most valuable companies.

From Publisher: Billboard



Fredonia schools recognized for music programs | News, Sports, Jobs - Observer Today

For the ninth year in a row, the NAMM Foundation has recognized the Fredonia Central School District for its outstanding commitment to music education with a “Best Communities for Music Education” designation. Only 4% of the nation’s school districts were honored with this designation.

Now in its 22nd year, this award recognizes school districts that have demonstrated exceptional efforts toward maintaining music education as part of their schools’ curriculum. This designation is meant to highlight the importance of supporting and expanding music education opportunities in our nation’s schools and acknowledging music’s vital role in student success.

Every year the National Association of Music Merchants collects data regarding public school music programs’ student participation in curricular and extracurricular music, pupil and staff achievements, community engagement, district support, and more. NAMM and the Center for Music Research at the University of Kansas compare this data across districts nationwide and come up with a list of the “Best Communities for Music Education.” The Fredonia School District is excelling in all of these categories.

Brad Zilliox, Fredonia’s Superintendent, shared, “Being relatively new to the district, the music program was repeatedly mentioned in my entry interviews as being a great source of pride for the entire school community. I have been impressed with how our department responded to the pandemic and continued to produce high quality results despite some of the limitations that were in place. It is clear that we have a talented group of students attached to all of our music offerings who excel in this subject area. We also recognize their achievements would not be possible without the support of our parents and families. I look forward to eventually seeing all of our groups in-person when we are back to our usual format for concerts and other music related events. Congratulations to all involved on a job well done!”

For more information on the Fredonia Central School District’s Music Department please visit their website at: tinyurl.com/FCSDMusic

There was some good news in regard to Thursday's updated COVID-19 numbers from the Chautauqua County Health ...

ASHVILLE — Two smaller solar projects have been given financial approval to be built in the town of ...

An additional 30 businesses have been approved to be recipients of a $10.5 million grant through the CARES Act, ...

From Publisher: observertoday.com



The Mitchells vs The Machines Cast Guide: What The Actors Look Like

The Mitchells vs. The Machines cast is loaded up with big name celebrities, so who are the main players? Produced by Oscar winners Phil Lord and Christopher Miller ( Spider-Man: Into the Spider-Verse ), The Mitchells vs. The Machines follows a family during a road trip from Michigan to California. Along the way, they get caught up in robot revolution and team up with their fellow humans.

The Mitchells vs. The Machines was directed by Mike Rianda, who co-wrote the animated comedy with Jeff Rowe. The storyline focuses on familial dynamics and all the personal quirks that make each individuals so unique. The filmmakers incorporated numerous Easter eggs for Netflix audiences to locate, and feature two different styles of animation for the humans vs. technology narrative.

Abbi Jacobson voices Katie Mitchell, an 18-year-old aspiring filmmaker who produces YouTube videos starring her pug, Monchi. During a tech uprising, she channels her inner action hero. Jacobson starred as Abbi Abrams in Broad City and portrayed Jessica Baiers in Neighbors 2: Sorority Rising . She also voices Bean in Disenchantment .

Danny McBride voices Rick Mitchell, Katie's father. He despises technology and isn't surprised by a robot uprising. McBride starred as Kenny Powers in Eastbound & Down and Neal Gamby in Vice Principals . He also portrayed Red in Pineapple Express and Jess Gemstone in The Righteous Gemstones .

Mike Rianda voices Aaron Mitchell, Katie's younger brother. He's a dinosaur enthusiast who doesn't have strong social skills. Aside from directing The Mitchells vs. the Machines , Rianda voiced Mike in Gravity Falls .

Eric Andre voices Dr. Mark Bowman, a tech entrepreneur who created PAL Labs. He fails with his safety precautions and inadvertently causes a Pal Max uprising. Andre hosted The Eric Andre Show from 2012 to 2020 and voiced Azizi in The Lion King (2019). He also voices Luci in Disenchantment and starred as Chris Carey in the 2021 Netflix movie Bad Trip .

Olivia Colman voices PAL, a virtual assistant operating system. She leads a revolution against her creator and all humans. Colman portrayed Doris Thatcher in Hot Fuzz and Queen Anne in The Favourite . She also stars as Queen Elizabeth II in The Crown and headlined The Father as Anne.

Chrissy Teigen voices Hailey Posey, the Mitchell's neighbor. Teigen is a Sports Illustrated Swimsuit model and currently hosts Lip Sync Battle . She also voiced Crystal in Hotel Transylvania 3: Summer Vacation .

Charlyne Yi voices Abby Posey, Hailey and Jim's daughter who forms a bond with Aaron. Yi portrayed Jodi in Knocked Up and Dr. Chi Park in House . She also voiced Ruby in Steven Universe and Pennywhistle in the 2020 movie Trolls World Tour .

From Publisher: ScreenRant



Billy Joel's daughter Alexa Ray reveals how the star reacted to her new song: 'He called me right

"He called me right away," the 35-year-old told the outlet. "He's pretty low-key, but he was like, 'Honey, this is great!' And I said, 'Oh you kind of sound more like my mother. So enthusiastic!'"

Back in 2015, Alexa Ray told Fox News that her father has greatly influenced her as she embarked on her own career as a solo artist.

"He can transcend genres and do of these different voices," she shared at the time. "He can do that more doo-wop, Frankie Valli and the Four Seasons sound that he does on '52nd Street,' but he can be more of a classic rock 'n' roll guy, which he does on 'Glass Houses.'"

"I learned about embracing all different genres as opposed to just getting attached to one. I just learned so much from him. He's like a musical encyclopedia," she added.

She shared it was Brinkley, 67, who encouraged her to be confident, as well as to embrace herself, inside and out.

"I've learned pretty much everything from my mom," said Joel. "She's taught me a lot about just taking care of yourself — skincare, great makeup tricks. She's very good at putting together that very effortless-style look… [And] she's got the best style. She's really the most stylish woman I know. I'm just trying to keep up!"

The Colts will be adding draft picks and rookie free agents to the roster in the coming days and they cleared out some new space for new arrivals on Wednesday. The team announced that they have waived tackle Elijah Nkansah, punter Austin Rehkow and tackle Casey Tucker. None of the three players saw game action [more]

Zoey's Extraordinary Playlist star Skylar Astin discusses his musical inspiration, Billy Joel. "I've been listening and performing Billy Joel long before I ever got into this professionally. I used to audition with New York State of Mind," says Astin. "He tells such a story and I sang all of his songs for so long. I even have this piano book from childhood on my piano still in my house." Astin describes meeting Billy Joel after seeing him perform and play piano in the play, "Spring Awakening." "He came backstage. He was in the dressing room... He said, 'Oh there's the piano man' and I literary just completely fan boy-ed out. I just couldn't even believe that Billy Joel knew who I was." Astin adds, "It's definitely a dream of mine to be able to play him in a biopic at one point. That's definitely a dream of mine that I want to put out there at some point."

(Toronto Maple Leafs) with a Goalie Save from Montreal Canadiens vs. Toronto Maple Leafs, 04/28/2021




'Port Authority' Trailer: Leyna Bloom and Fionn Whitehead's Ballroom Love Story

Leyna Bloom and Fionn Whitehead star in a New York City love story set amid the ballroom scene in the drama Port Authority , and ET can exclusively debut the trailer.

Whitehead plays Paul, who finds himself in NYC with nowhere to turn after being kicked out of his home. A chance encounter with Wye (Bloom), a trans woman of color and ballroom regular, gives way to a blossoming romancing as the couple navigates chosen family and the secrets of Paul's past. Watch the trailer above.

"It has always been my dream to have the chance to represent the ballroom community on the big screen," Bloom tells ET. (The actress, who next stars on Pose , made history as the first trans woman of color to appear in the Sports Illustrated Swimsuit Edition .) "Ballroom means so much to LGBTQI+ youth of color and my personal evolution as a trans person. I was raised by ballroom, and this has been one of my wildest dreams come true, being able to pay homage to so many trans women who came before me."

"I am so thrilled to see our community loved and celebrated on the big screen," she adds. "I hope that 100 years from now when future queer youth watch this, they can feel celebrated past, present and future."

Port Authority counts Martin Scorsese as an executive producer and premiered at the Cannes Film Festival. For writer-director Danielle Lessovitz, "I wanted to honor a community that for me, is the most beautiful expression of what it means to be American, while at the same time, showing how the inability to love and be loved -- openly and honestly -- is its own type of oppression."

Jack Dylan Grazer and Fionn Whitehead Hold Rainn Wilson Captive in 'Don't Tell a Soul' Trailer (Exclusive)

From Publisher: cbs8.com



Natori to Expand in Europe With Wholesale Operations – WWD

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From Publisher: WWD



MBtv: Sex sells - China farm region becomes 'lingerie capital' |

The flat farming region of Guanyun is China’s self-proclaimed “Lingerie Capital”, where sewing machines hum in village-level micro-factories to meet up to 70 percent of the fast-growing domestic demand. Millions more items are exported annually in a textbook example of the ability of internet-enabled Chinese entrepreneurs to profit from even the most off-the-wall idea.

From Publisher: Macau Business



Online Lingerie Market Revenue Analysis Report with Future Business Scope and New Investment

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From Publisher: The Courier



Falkirk lingerie store Miss Forsyth celebrates 140 years in business | The Scotsman

Remarkably, the shop remains based in the same Lint Riggs premises where it began in 1881 and has seen countless stores come and go in the town over the decades.

Current owner Sarah Williamson is one of just five women to have held responsibility for managing the business, having taken over from her mum, Jennifer Moodie.

An employee, known as Mrs Kingston, then took over following the siblings' retirement and ran the shop until she turned 80 before Mrs Moodie bought the business in 1984.

What matters most to the businesswoman, however, is the premises has reopened following the latest lockdown, meaning she and her staff can continue to create more Miss Forsyth history.

A "boom" in business in the 1980s resulted in Miss Forsyth opening a second store in Perth the following decade.

Sarah, who began working at the store aged 14 as a Saturday girl, said: "With everything that's going on, trying to do anything is such a thought.

"The big thing this year is we've survived this pandemic. The shop's also survived two world wars – there aren't many shops that can say that these days.

"The customers have been immensely important. That's what's kept me going, especially over the last year. It's been so tempting to call it a day.

"I'd like to thank our customers, who are generations' worth. Girls come in with their gran and mum to get their first bra. It's like an institution."




Global Maternity and Nursing Lingerie Market Snapshot On Industry Trends And Opportunities 2026

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90 Day Fiancé: Molly Hopkins Pitches Her Dream Reality Show Concept

Molly also opened up about guest-starring on Little Women Atlanta. Molly revealed that show star Juicy has been a beloved client of Livi Rae Lingerie for some time. Molly enjoys making Juicy look and feel good. " I know her favorite color. She comes there because she feels safe, and she also feels important," Molly said. She added that having a good relationship with her customers is important to her because the 90 Day Fiancé star wants women to both look good and feel confident. " I think that that's two really great things to have in your relationship with a bra fitter. If someone's going to help you dress up sexy, and you're not sure what to do, then you go to the experts. And that's us; we can stand very confidently in that space ," she added.

Molly revealed that there are a number of celebrity clients she would love to dress because sometimes she sees how poorly their bras fit on the red carpet. Her dream client would be Porsha Williams from Bravo's The Real Housewives of Atlanta . Molly cited Posha as an amazing woman who is truly dedicated to helping other people. " And she has a great body, " Molly added. " She's thick. She's got great boobs. I would love to bra fit her and get her into some sexy gear. " The TLC star said she would be delighted to one day work with Porsha on a future collaboration.

From Publisher: ScreenRant



Online Lingerie Market Continues Rapid Growth | Study Reveals Market Size for Emerging Segments



Global Men’s Underwear and Women’s Lingerie Market Growth Factor with Key Drivers

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Men’s Underwear and Women’s Lingerie Market competition by top manufacturers/Key player Profiled:
Victoria’s Secret (L Brands), Calvin Klein, Fruit of the Loom (Berkshire Hathaway), Hanky Panky, Cass and Company, Commando LLC

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Global Online Lingerie Market In-deep Analysis And Experts Review Report 2021-2024 – Clark

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Victoria?s Secret
PVH
Hanesbrands
Fruit of the Loom
Aimer
Fast Retailing
Triumph
Huijie
Jockey International
Wacoal Holdings
Cosmo-lady
Gunze
Embry Form
Calida
Oleno Group
Vivien
Tutuanna
Sunny Group
Miiow
GUJIN
Hop Lun
BYC
Sunflora
Good People
P.H. Garment
SBW

Segments 1 and 2: Market definitions, Online Lingerie market scope, classifications, applications, market concentration, and market size calculations are analyzed in this segment. In addition, the market presence across different regions and market statistics for these regions will be assessed from 2015 to 2019. Online Lingerie Production and growth rates are analyzed in each region. It also provides comprehensive coverage of industry policies and plans, market drivers, constraints, and the latest industry news.

Segments 3 and 4: Online Lingerie Industrial Chain Structure, Production Volume, Raw Material Costs, Manufacturing Costs, Market Leading Online Lingerie Players, Supplier, and Buyers are investigated. Online Lingerie Market classifications by type include growth rate, price analysis, value and quantity analysis, and market share.

Segments 5 and 6: This segment evaluates consumption and market share based on Online Lingerie applications. This segment also surveys region-based Online Lingerie production and gross margins.




Thursday, April 29, 2021

The Magic Eastern Ensemble Combines Traditional Chinese Instruments With Contemporary Pop Music |

The Magic Eastern Ensemble is performing at Aurora Theatre Saturday, May 1 in a concert called “ We Are The Young .” The concert will feature traditional Chinese instruments like the erhu, guzheng, and dizi, in a musical blend of new Chinese folk music, J-pop, and English and American pop music. The ensemble's Director Yao Lu and Aurora Theatre's Director of Education Renita James joined "City Lights" host Lois Reitzes to talk about what to expect from this unique performance and other upcoming highlights from Aurora.

"I think the traditional music instruments, it is already a sense of history. But I wanted to… make it not too difficult to attract the attention and recognition of the audience, who never knew about this type of instrument," said Lu. "We hope we will have more young audiences who will enjoy the music, as we play it."

Lu invited the Ensemble's lead ehru player Cindy Fang to join the conversation, who added, "[The erhu] is a two-stringed bow musical instrument, sometimes known in the Western world as a Chinese violin… It is also combined with traditional and contemporary music styles and arrangements, such as in pop, rock and jazz."

The performance will be an indoor concert, Saturday, May 1 at 8 p.m. Guests will be seated in pods, and all transactions and services conducted in touchless arrangements. Tickets can be purchased in advance at auroratheatre.com .

From Publisher: 90.1 FM WABE



Tibetan pop music group performs Tibetan-style song - CGTN

Tibetan pop music group ANU performed a Tibetan-style song "Fly" at the music festival celebrating the production of CGTN's upcoming documentary "Music Voyage." Take a listen!

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Tibetan pop music group sings to send blessings - CGTN

Tibetan pop music group ANU performed a Tibetan-style song, "1376," at the music festival celebrating the production of CGTN's coming musical road trip "Music Voyage." "1376" is a homonym for "may all your wishes come true" in Tibetan. Take a listen!

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Global showcase for Griffith pop music student – Griffith News

The former First Lady of the United States, Michelle Obama , will showcase a song written by Griffith University popular music student Cat Gallagher as part of her annual College Sign Up Day campaign.

Penned for an online collaborative project between music students from the Queensland Conservatorium and Vanderbilt College in the US, the song will be featured on Mrs Obama’s social platforms to mark National College Signing Day on 1 May.

Mrs Obama launched the signing day celebrations in 2014 as part of her Better Make Room initiative to encourage students to pursue higher education.

Her social accounts have more than 80 million followers – giving the young songwriter a remarkable platform for her work.

A series of student performances will feature alongside world-famous acts like Conan O’Brien, The Weeknd and Kelly Rowland.

“This song is very close to my heart, so to be able to share it with the world is incredible.

“This is a massive opportunity for me to reach out to a global audience and expand my musical community.”

During COVID lockdowns last year, a number of Queensland Conservatorium popular music students collaborated with their peers across the globe, creating new music with singer-songwriters at the Vanderbilt College Blair School of Music in Nashville.

"Her Troubles, His Pain" was a joint effort between Cat and Cam Robinson, a human resources and organizational development major at Vanderbilt College. The track was mixed by fellow popular music student Liam Brimble .




Watch Opelika High's Vibe students play Journey's "Who's Crying Now" and other pop

The Opelika High School Vibe will be featured in the virtual concert series as part of the MIC CHECK 2021 event put on by Troy University on Friday and Saturday.

Students from the Opelika High School Vibe will perform covers and original songs at Troy University's MIC CHECK 2021 virtual concert event Friday and Saturday premiering at 7 p.m.

Opelika High School guitar instructor Patrick Bruce said about 15 students are currently in the Vibe, the high school's popular music performance group, that are able to practice and perform various music talents from vocals to guitar to drums.

"I think [my students] will be able to see other kids their age making music, and they'll know how much effort it takes to put something together like this," Bruce said. "And they'll get critiqued by professionals in the music industry, so they'll get some feedback on where they stand compared to them. Also, it's just fun."

"We used to go to Troy and hang out all day, play music and get critiqued by professionals," Bruce said. "This year, they came to Opelika high School about a month or so ago and we set up my room for recording and they videoed us."

Bruce said his students would be performing covers they picked to play like Journey's "Who's Crying Now" along with original songs like one written by Opelika High School senior Lorenzo Farrow. Overall, Bruce said the MIC CHECK gave his students a goal to strive for while getting the opportunity to receive constructive criticism from music professionals.

Deirdre Freeman walks the mile from her home, lugging a large, colorful painting and tote bag with her hammer and nails. Her destination is a telephone pole at a busy intersection, where she will hang her largest "heartwork" painting yet.

"The camaraderie they have, and the family we have as a group, is a good thing," Bruce said. "They look forward to coming to school, and doing something like this gave them a goal and something to shoot for."

Among the music professionals to provide feedback and teach lessons to the students performing is Michael Wilk, the keyboardist for the band Steppenwolf who has collaborated on musical projects with members of Fleetwood Mac and Three Dog Night.

From Publisher: OANow.com



Billboard Awards: 16 nods for Weeknd, 6 for Morgan Wallen | Music | dailyprogress.com

The Weeknd was snubbed by the Grammys but he's the leading nominee at the 2021 Billboard Music Awards.

Country singer Morgan Wallen wasn't allowed to compete at this month's Academy of Country Music Awards because the singer was caught on camera using a racial slur earlier this year, but he's one of the top nominees at the 2021 Billboard Awards.

NEW YORK — The Weeknd was snubbed by the Grammys, but he's the leading nominee at the 2021 Billboard Music Awards, where rappers Pop Smoke and Juice WRLD earned multiple posthumous nominations, including top artist, and disgraced country singer Morgan Wallen is a six-time nominee.

The Weeknd earned 16 nominations, including top male artist, top R&B artist, top Billboard 200 album for "After Hours" and top Hot 100 song for "Blinding Lights," the No. 1 song of last year. DaBaby — thanks to his own hit "Rockstar" and his guest appearance on Jack Harlow's "What's Poppin" — is second with 11 bids, and he will battle himself in categories like top rap song, top streaming song and top collaboration.

Others battling The Weeknd, Pop Smoke and Juice WRLD for top album include Lil Baby's "My Turn" and Swift's "folklore," which last month won the star her third album of the year Grammy.

Morgan Wallen wasn't allowed to compete at this month's Academy of Country Music Awards because the singer was caught on camera using a racial slur earlier this year, but he's one of the top nominees at the Billboard Awards. His six nominations include top song sales artist, top country artist and top country album for "Dangerous: The Double Album," which is currently No. 3 on the pop charts and continues to sell extremely well despite Wallen's crisis moment. He's even a double nominee in top country song, competing with the hits "Chasin' You" and "More Than My Hometown."

Wallen is a contender because the Billboard nominees are based on album and digital sales, streaming, radio airplay and social engagement, and they "are not chosen by a voting committee or membership organization," dick clark productions said.

The producers said though Wallen is a multiple nominee, they won't allow him to participate in the show.

From Publisher: The Daily Progress



Advanced BTS: California university to host scholarly online conference focusing on Korean boy

An ARMY plans to assemble on Saturday, May 1, and Sunday, May 2 – one that carries no weapons, requires no minimum enlistment period and marches to a decidedly pop-music beat.

California State University, Northridge will host an online conference about all things BTS, the megapopular Korean boy band, this weekend.

Fans, who call themselves "ARMY" or Adorable Representative M.C. for Youth, will turn out in droves for the conference with more than 900 attendees expected, according to event organizer Professor Frances Gateward.

BTS, an acronym for Bangtan Sonyeondan or Bulletproof Boy Scouts, debuted in 2013 and consists of seven members. While primarily gaining fame in Korea and other parts of Asia early on in their career, BTS garnered a worldwide following, performing in places ranging from the Rose Bowl in Pasadena to King Fadh International Stadium in Riyadah, Saudi Arabia. The group has won dozens of awards and was the top-grossing touring group of 2019 according to Billboard, earning $196 million from Nov. 1, 2018 to Oct. 31, 2019.

Despite their worldwide popularity, there are many people who still consider the group just an average boy band, but fans of BTS aren't of the same opinion.

"People are surprised to find out that the median age of a BTS fan is 30 to 40," Gateward said. "It's not 12-year-old girls."

Other groups play socially conscious music and perform charity work as well, but BTS has gained fans at a rate unlike many other groups in the world. The band has more than 30 million followers on Twitter, and sold out one performance at the Rose Bowl in 90 minutes.

"They present the image of accessibility," said Analisa Venolia, a member of the ARMY who plans on attending the conference. "They interact with fans in a way that I'm not used to seeing from a lot of American artists so that makes them really unique and you feel like you get to know the members."

Venolia pointed to Weverse, a Korean social media app designed to facilitate interactions between fans and their favorite artists, as one way in which the band connects with its ARMY. Each member of the group has their own Weverse account, so fans can interact with them more individually than as a group. Members of the ARMY begin to get a sense of their individual personas further outside the context of the band than seen on more mainstream apps like Twitter and Instagram.

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From Publisher: The Mercury News



Girl in red on new album and how her gen of pop are tired of "meaningless lyrics" | Girlfriend

When you think of the artists whose songs are on your go-to moody indie-pop playlists aptly titled music to soundtrack me screaming in the woods , and the ones featured in the dream-pop meets queer teen memes about serotonin and therapy in the many indie-pop Facebook groups, you can't not think of the 22-year-old Norweigan artist girl in red.

The singer-songwriter rose to fame in recent years with homemade bedroom pop songs about queer romance, unrequited love, and mental health, and is back at it again with her 2021 feature album if i could make it go quiet .

In the lead up to her new album, which she says "sheds light on the darkest parts of [her] mind" and "lets everyone in," Girlfriend spoke to the incredible, showstopping, never-the-same artist about her last 12 months, why artists her generation are so confessional, and how she's become the role model she always wanted to be.

Growing up in Horten, a small seaside town in Norway with only 26,000 people, girl in red, real name Marie Ulven, was born to be a star.

Surrounded by nobody like her and with no real LGBTQIA+ representation as a kid, she, like many rural queer Aussie teens, turned to YouTube personalities and the internet to be inspired and find a community.

But now, she *is* the queer voice that people younger than her turn to. Back in World War II, queer men would use the term "friend of Dorothy" as a euphemism for being gay to allow themselves to freely find each other and exist in an age of queer oppression. Girl in red's name carries a similar legacy: now, teens on TikTok jokingly ask, "do you listen to girl in red?" as a way to tell if someone identifies as queer.

"It's so rare… I'm just thinking about anyone who's ever gonna use it or have used it. I'm like, 'good for you! You go.'"

And, really, it isn't hard to see how she became a signifier for queer teen identity and young first love when you look at her music.

But, at the beginning of 2020, something changed. As the world began to tackle a global health crisis, Marie was dealing with a "health anxiety" of her own. The Serotonin singer was diagnosed with Obsessive-Compulsive Disorder (OCD).

From Publisher: Girlfriend



Top Hollywood talent agency to represent ‘pop music superstar’ JJ Lin | The

The Hollywood Reporter in an “exclusive” report says the "Chinese Pop Music Superstar JJ Lin" has signed with United Talent Agency (UTA), one of the biggest in Hollywood.

UTA announced that it signed Lin with an Instagram post on Wednesday (Apr 28), noting that his music has reached more than 121 billion streams all over the globe via online platforms, and that he has also received nearly 400 music awards all over Asia.

"His debut album, Music Voyager, won him the award for 'Best New Artist' at the Golden Melody Awards.

“He has completed four world tours and more than 130 shows, with 66 shows from his recent Sanctuary World Tour selling out within 10 minutes of release," UTA's post read.

The post also mentioned the international artists the superstar has collaborated with, including Hans Zimmer, Jason Mraz, Ayumi Hamasaki, Steve Aoki and Jackson Wang.

On his English album, Like You Do, Lin sings a ballad with UK singer Anne-Marie called "Bedroom." Take a listen here .

The Hollywood Reporter in its report on Apr 27 said Lin has been named one of Forbes Asia's 100 Digital Stars and appeared as a guest on The Voice in China.

He is also active in gaming, having launched the professional esports team Team Still Moving under Gunfire (Team SMG) in Southeast Asia and China.

The singer also posted about his new representation, writing “Happy to be part of the UTA family! @unitedtalent" on Instagram .

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International Jazz Day 2021: How to celebrate in Phoenix and beyond

"They had 7,000 people out there at about 8 o'clock in the morning," he says. "A phenomenal sight."

A year later, Jones — a music educator, who plays saxophone and keyboards — organized the Valley's first International Jazz Day, held at Cityscape.

It's Jones' hope that a day set aside to recognize the genre could help elevate its profile and attract new audiences in an era where its mainstream popularity has been eclipsed by hip-hop, R&B and rock.

"Jazz was an art form created here in America that's been exported all around the world," he says. "And now 1% to 2% of music sales are jazz."

As Blaise Lantana, a Valley musician who serves as both music director and on-air personality at KJZZ-FM, sees it, anything that recognizes jazz in the 21st Century is a step in the right direction.

Jones and his International Jazz Day AZ Foundation will present a Scottsdale Jazz Day Festival at the Hotel Valley Ho while the Nash in downtown Phoenix hosts an outdoor show by the Max Beckman Quartet.

International Jazz Day was launched in 2011 by Herbie Hancock, the United Nations Educational, Scientific and Cultural Organization (UNESCO), and the Thelonious Monk Institute of Jazz.

"Herbie realized that in America jazz is being swept under the carpet unless you're an aficionado, you're an educator or you're in school playing this music," Jones says.

"So he went to Tom Carter, the executive director at the Thelonious Monk Institute, and they said, 'We have to do something. This genre is not getting any publicity. It's losing ground.'"

From Publisher: The Arizona Republic



Kandi Burruss Flaunts A Gorgeous Cleavage In This Video - Check It Out Here | Celebrity Insider

Kandi Burruss is flaunting a gorgeous cleavage in this video that she shared on her social media account. You can also check it out below.

'Today started off right! Thank you, @georgemiguelc & @theglamfather, for getting me all the way together! Of course, I was styled by @therealnoigjeremy,' Kandi said.

RELATED: Kandi Burruss Celebrates Her Half Sister's Birthday - Fans Are Surprised To See Rebekah

One other follower said: '@kandi, I swear you are ageing backwards, Sis😍😍' and someone else said: 'I love the talks being posted between you and Don Juan. You are a true boss and a class act. I love to see it.'

One followewr said: 'Kandi!! Hi, y'all! I wrote a diverse children's book: "Black, Brown or White We All Feel", Available on Amazon. Reinforces positive behaviour in our children. Inspired by my bullied 5-year-old,' and a fan posted this: 'yes kandi, you always been beautiful, but girl you been serving lately.'

Kandi Burruss addressed the RHOA reunion part one, which will take place this week. Check out the post that she shared in her social media account below.

'They said the theme is Burlesque, 50 shades of grey, dungeon vibes. 😜You know I love a theme! Reunion part one is Sunday. Get ready!' Kandi captioned her post.

Kandi Burruss shared some pics in which she's making some really funny faces. Fans cannot have enough of her, and you can also check out her looks below.

'I can not control my faces! These looks at the gift exchange for @cynthiabailey's #FriendsMas are hilarious! 🤪,' Kandi captioned her post.




Princess Diana's genius fashion hack revealed | HELLO!

Princess Diana was one of the most photographed women in the world and she never failed to look incredible, whether she was wearing elegant dresses or casual gym gear.

But on the many occasions that she would don her finest wear for formal events, Diana was rarely seen without a small matching clutch bag in tow – and there was a very good reason for it.

The late princess would use her clutch – which always matched her evening dress – to cover her chest so she didn't reveal more than she wanted when she exited a car.

Diana even coined a clever name for her fashion hack, according to handbag designer Anya Hindmarsh, who previously told The Telegraph : " We used to laugh when we designed what she called her 'cleavage bags', little satin clutches which she would cover her cleavage with when she stepped out of cars."

"She was a very loyal customer and a lot of fun," Anya added. "She would come and see us with no bodyguards or any fuss."

MORE:  Prince William and Prince Harry agree on beautiful tribute for late mother Princess Diana

The Duchess of Cambridge is often spotted holding her bag in her left hand so that she can keep her right hand free to greet and shake hands with guests.

The Queen also reportedly uses her famous Launer purse to send secret messages to her staff. When Her Majesty switches her bag from one arm to the other, it's said to be her way of silently indicating that she'd like to wrap up her current conversation.

From Publisher: HELLO!



How SARS-CoV-2 first adapted in humans | Science

Viruses need entry proteins to penetrate the cells where they will replicate. The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) version is called the spike or S protein. The S protein, also the target of the current vaccines, is quickly adapting to its new human hosts. It took its first major step in this direction early in 2020, when its amino acid 614 (of 1297) changed from an aspartic acid (D) to a glycine (G). Viruses bearing this D614G mutation transmit among humans more rapidly and now form the majority in circulation. On page 525 of this issue, Zhang et al. ( 1 ) use careful structural analyses to reveal how D614G changed the S protein to accelerate the pandemic.

This fix solved a technical problem, but it deepened a mystery. Although a number of distantly related coronaviruses carry furin cleavage sites at their S1-S2 boundaries, the SARS-CoV-1 S protein, and those of all known bat-derived viruses from the same Sarbecovirus lineage, lack this site. Instead of being cleaved in virus-producing cells, their S proteins are cleaved by different proteases while the virus is engaging ACE2 in the next, yet-to-be-infected cell ( 5 ). As it happened, furin-site mutations that improved SARS-CoV-2 S-protein function in pseudoviruses allowed the modified S protein to work with these later-stage enzymes, just like the SARS-CoV-1 version. Why then did the SARS-CoV-2 furin site persist, even though it made infection in cell culture less efficient? Indeed, viruses passaged in culture regularly lost this site. Does it somehow improve viral transmission? Would it eventually disappear over the course of the pandemic?

The underlying mechanism for this fitness advantage remained a point of controversy. Here, a second unusual property of the S protein, in this case shared with SARS-CoV-1, became relevant. The SARS-CoV-2 S protein, like most entry proteins of viruses with a lipid membrane, assembles into trimers. Typically, during the process of virion assembly, viral entry proteins subtly change their conformations, but it is unusual for these proteins to break their three-fold symmetry before they bind their receptor. However, the mature SARS-CoV-2 S protein often assumes an asymmetrical arrangement whereby one of its three RBDs assumes an open or "up" conformation ( 1 , 9 ). Only RBDs in this up conformation can bind ACE2. Once it does so, the S1 domains dissociate from S2, and S2 undergoes a pronounced rearrangement to a "postfusion" state. The released energy of this rearrangement drives the viral and cell membranes to fuse and gives the virus access to the cell interior.

The Gly 614 (G614) mutation in spike (S) increases ordering of the 630 loop compared with wild-type Asp 614 (D614). This prevents the premature S1 shedding often seen with wild-type S proteins, ensuring that more S protein remains in a fusion-ready "one-up" state, with one receptor binding domain (RBD) exposed within the trimer, ready to bind angiotensin-converting enzyme 2 (ACE2) on host cells, increasing infection efficiency.

To cut through this controversy, Zhang et al. solved the structure and provided detailed analyses of both D614 and G614 S proteins in multiple states. They first noted that, as they and others had previously observed, the loss of D614 in S1 breaks an ionic bond to a proximal lysine, K854, in S2 ( 9 ). Loss of this salt bridge is initially counterintuitive because it would loosen the association between S1 and S2, although it might ease the movement of the RBD into the up configuration. However, the structures from Zhang et al. show that a major difference between S proteins with and without D614G is the visibly greater ordering in G614 S proteins of a region spanning residues 620 to 640, which the authors call the "630 loop." This loop is just downstream of G614. It is therefore possible that either the loss of the D614-K854 salt bridge, or the greater backbone flexibility that a glycine affords, helps the 630 loop nestle more tightly in a canyon formed by two larger S-protein domains (the amino-terminal domain and carboxyl-terminal domain 1). Regardless, this loop is found in a more rigid and stable arrangement between these domains when residue 614 is a G than when it is a D.

The key is that both the RBD-up conformation and dissociation of S1 from S2—enabled by furin cleavage—require disordering of the 630 loop. Thus, the RBD-up conformation can be more easily accessed with the original D614 S protein, but once this conformation is achieved, this S protein is more likely to fall apart entirely owing to premature shedding of its S1 domain. Conversely, with G614, more energy is required to achieve a one RBD–up state, but dissociation of S1 from S2 also becomes less favorable because the remaining folded 630 loops continue to hold the trimer together. Thus, the D614G variants have more S proteins in the up orientation because the next, irreversible step toward inactivation is slower. Infection with D614G is more efficient because it prevents premature S1 shedding (see the figure).

From Publisher: Science



Structural impact on SARS-CoV-2 spike protein by D614G substitution | Science

Throughout the COVID-19 pandemic, epidemiologists have monitored the evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with particular focus on the spike protein. An early variant with an aspartic acid (D) to glycine (G) mutation at position 614, D614G, rapidly became dominant and is maintained in current variants of concern. Zhang et al. investigated the structural basis for the increased spread of this variant, which does so even though it binds less tightly to the host receptor (see the Perspective by Choe and Farzan). Structural and biochemical studies on a full-length G614 spike trimer showed that there are interactions not present in D614 that prevent premature loss of the S1 subunit that binds angiotensin-converting enzyme 2. This stabilization effectively increases the number of spikes that can facilitate infection.

Substitution for aspartic acid (D) by glycine (G) at position 614 in the spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) appears to facilitate rapid viral spread. The G614 strain and its recent variants are now the dominant circulating forms. Here, we report cryo–electron microscopy structures of a full-length G614 S trimer, which adopts three distinct prefusion conformations that differ primarily by the position of one receptor-binding domain. A loop disordered in the D614 S trimer wedges between domains within a protomer in the G614 spike. This added interaction appears to prevent premature dissociation of the G614 trimer—effectively increasing the number of functional spikes and enhancing infectivity—and to modulate structural rearrangements for membrane fusion. These findings extend our understanding of viral entry and suggest an improved immunogen for vaccine development.

( A ) The full-length SARS-CoV-2 S protein carrying either D614 or G614 was extracted and purified in detergent n-dodecyl-β- d -maltopyranoside (DDM) and further resolved by gel-filtration chromatography on a Superose 6 column. The molecular weight standards include thyoglobulin (670 kDa), γ-globulin (158 kDa), and ovalbumin (44 kDa). Peak I is the prefusion S trimer; peak II is the postfusion S2 trimer, and peak III is the dissociated monomeric S1. The insets show peak fractions that were analyzed by Coomassie-stained SDS-PAGE. Labeled bands are S, S1, and S2. Fr#, fraction number. ( B ) Binding analysis of fractions of peak I in (A) with soluble ACE2 constructs by BLI. The purified S proteins were immobilized to AR2G biosensors and dipped into the wells containing ACE2 at various concentrations (5.56 to 450 nM for monomeric ACE2; 2.78 to 225 nM for dimeric ACE2). Binding kinetics was evaluated using a 1:1 Langmuir binding model for the monomeric ACE2 and a bivalent model for dimeric ACE2. The sensorgrams are in black and the fits in red. Binding constants are also summarized here and in table S1. All experiments were repeated at least twice with essentially identical results. K D , dissociation constant (binding affinity); RU, response unit.

( A ) Three structures of the G614 S trimer—representing a closed, three RBD–down conformation; an RBD-intermediate conformation; and a one RBD–up conformation—were modeled on the basis of corresponding cryo-EM density maps at 3.1- to 3.5-Å resolution. Three protomers (a, b, and c) are colored in red, blue, and green, respectively. RBD locations are indicated. ( B ) Top views of the superposition of the three structures of the G614 S in (A) in ribbon representation, with the structure of the prefusion trimer of the D614 S (Protein Data Bank ID: 6XR8) shown in yellow. The NTD and RBD of each protomer are indicated. Side views of the superposition are shown in fig. S8.

To examine the structural changes resulting from the D614G substitution, we superposed the structures of the G614 trimer onto the D614 trimer in the closed conformation, aligning them by the invariant S2 ( Fig. 2B ). A shift by a clockwise, outward rotation of all three S1 subunits, relative to the D614 structure, is evident even for the G614 trimer in the closed conformation. A similar shift was also observed in the RBD-intermediate and RBD-up G614 structures. Thus, the D614G substitution has led to a slightly more open conformation than that of the D614 trimer, even when all three RBDs are down. The D614G change has apparently also rigidified a neighboring segment of CTD2, residues 620 to 640, which we designate the 630 loop. This loop inserts into a gap, slightly wider in the G614 than in the D614 trimer, between the NTD and CTD1 of the same protomer ( Figs. 3 and 4 ). The 630 loop is disordered in the closed D614 trimer (fig. S10) because the gap is too narrow for it to insert. The closed D614 trimer thus has three ordered FPPRs and three disordered 630 loops, whereas the closed G614 trimer has three structured 630 loops along with three ordered FPPRs. In the two conformers with one partly or fully open RBD, the two segments are disordered in the RBD-shifted subunit, and their central parts have difficult-to-model densities in one other subunit. The third pair appears well ordered throughout ( Fig. 3 ). Thus, the opening of the RBD in the full-length G614 trimer correlates with a displacement of the 630 loop and the FPPR having moved away from its position in the D614 trimer.

( A ) (Top) The structure of the closed, three RBD–down conformation of the D614 S trimer is shown in ribbon diagram with one protomer colored as NTD in blue, RBD in cyan, CTD1 in green, CTD2 in light green, S2 in light blue, the 630 loop in red, and the FPPR in magenta. (Bottom) Structures of three segments (residues 617 to 644) containing the 630 loop in red and three segments (residues 823 to 862) containing the FPPR in magenta from all three protomers (a, b, and c) are shown. The position of each RBD is indicated. ( B to D ) Structures of the G614 trimer in the closed, three RBD–down conformation, the RBD-intermediate conformation, and the one RBD–up conformation, respectively, are shown, as in (A). Dashed lines indicate gaps.

( A ) A close-up view of the region near the residue 614 with superposition of the G614 trimer structure in green (CTD2) and magenta (FPPR) and the D614 trimer in yellow, both in the closed prefusion conformation. Residues G614, D614, and two K854s from both structures are shown in stick model. The direction of the three-fold axis of the trimer is indicated. ( B ) Location of the 630 loop in the S trimer. The 630 loop is highlighted in red, the NTD in blue, the CTD1 in green, the CTD2 in light green, the S2 in light blue, and the FPPR from a neighboring protomer in magenta. The S1-S2 boundary and the nearest ordered residues Thr 676 from S1 and Ser 689 from S2 are all indicated. A strand from the N-terminal end of S2, packed in the CTD2, is highlighted in purple. ( C ) A view showing that the 630 loop wedges between the NTD and the CTD1 and pushes them apart. ( D ) Packing of the 630 loop against the hydrophobic surface formed by residues Val 595 , Val 597 , Val 610 , Tyr 612 , Val 642 , and Ile 651 from the CTD2 and Pro 295 from the NTD. Residues Trp 633 and Val 635 from the 630 loop contribute to this interaction.

CTD2 is formed by two stacked, four-stranded β sheets, with a fifth strand in one sheet contributed by the connector between the NTD and RBD. In the other sheet, an interstrand loop contains the S1-S2 cleavage site, and thus one strand is the N-terminal segment of S2 ( Fig. 4B ). In the G614 trimer, one side of the 630 loop packs along a long hydrophobic surface, largely solvent-exposed in the D614 trimer, formed by residues on the upward-facing surface of the CTD2 along with Pro 295 from the NTD ( Fig. 4D ). Trp 633 and Tyr 636 of the 630 loop appear to contribute to this interaction. S1 dissociation from S2 requires breaking the S2 strand from the second β sheet. An ordered 630 loop that stabilizes the CTD2 by closing off an exposed, hydrophobic surface may retard S1 shedding, thereby enhancing the stability of a cleaved S trimer. We note that the density for a fatty acid ligand making contacts with the neighboring RBDs in the D614 trimer is absent in all of the G614 reconstructions (fig. S11C) ( 37 ), which suggests that the ligand is not required for three RBDs to adopt the down conformation.

To test the impact of the 630 loop on S1 shedding and membrane fusion, we generated five S mutants, each containing a single-residue change either in the 630 loop (W633A, R634E, V635K, and Y636A) or the CTD2 hydrophobic surface (V610K) in the G614 sequence. These mutants expressed the same levels of S, with similar extents of cleavage between S1 and S2, as expected (fig. S12A). When detected by monoclonal antibodies using flow cytometry, mutants V610K and W633A showed markedly lower binding of RBD-specific antibodies [REGN10933 and REGN10987; ( 38 )] and of an NTD-specific antibody [4A8; ( 39 )] than the parental G614 S, whereas binding to an S2-specific antibody [0304-3H3; ( 39 )] was slightly higher (fig. S12B). These results are consistent with the hydrophobic interactions between the 630 loop and CTD2 stabilizing the cleaved S1-S2 complex and preventing S1 dissociation. The mutant V635K had wild-type phenotypes in these assays, likely because V635 does not make any direct contact with the CTD2. The mutants R634E and Y636A showed intermediate levels of antibody binding because Y636 appears to contribute less to the 630 loop–CTD2 interaction than W633, and R634 may help maintain the loop's overall shape for inserting between domains. Likewise, a similar pattern was observed with these mutants in the cell-cell fusion assay, except that Y636A showed substantially weaker fusion activity than R634E (fig. S12C). Thus, key residues important for stabilizing the S trimer structure appear critical for membrane fusion activity, as premature dissociation of S1 would lead to inactivation of the S trimer.

From Publisher: Science



Digital PCR Detection of mtDNA/gDNA Ratio in Embryo Culture Medium for | PGPM

Digital PCR Detection of mtDNA/gDNA Ratio in Embryo Culture Medium for Prediction of Embryo Development Potential

Noninvasive embryo screening technology is a new method to evaluate the contents of metabolites and nutrients in embryo culture medium. During the development of embryo, the contents of mitochondrion in the blastomere decreased gradually, and the contents of cell-free mtDNA in the corresponding embryo medium increased. It has been studied that the ratio of mtDNA/gDNA in embryo culture medium can be used to predict embryo development, but its predictive value is controversial, while the positive detection rate of gDNA is low, and a more accurate technology is to be found for verification. In this study, we used digital PCR system to explore the relationship between cell-free mtDNA in culture medium and the development potential of embryo. The mtDNA/gDNA ratio is associated with the cleavage stage embryo rating, fragmentation, and developmental state, and correlates with the blastocyst rating. According to our research, shortcomings are still associated with using only this indicator to predict embryonic development potential.

New techniques to screen for the highest quality embryos for transfer can reduce transfer embryo numbers, improve pregnancy outcomes, and reduce the risk of multiple births. This topic has become a focus of assisted reproductive technology (ART) research. Selective single blastocyst transplantation can improve pregnancy rates and reduce the risk of multiple pregnancies. 1 Embryos during the cleavage and blastocyst phases have been rated during in vitro fertilization (IVF) using morphological scoring methods. Some of these screening metrics have been applied to predict developmental potential including embryonic development speed, degrees of uneven blastomere cleavage, fragment content, inner cell mass (ICM), and trophectoderm (TE) levels. 2–6 However, that the best quality embryos will be selected for transfer is not guaranteed. Because morphology can appear normal, morphological screening cannot detect genetic defects, nor can it completely ensure optimal embryo development potential. Traditional observation at a fixed time point will miss some important parameters and key events during embryonic development. These parameters are all related to embryonic development potential, and to achieve standardization by relying on subjective differences in artificial observation scores is difficult. 7–9

After ICSI fertilization, embryos were cultured to the blastula stage in Sydney IVF Cleavage Medium (Cook Medical Inc., Bloomington, IN, USA) then transferred to blastocyst culture medium for embryo exchange on day 3. The cultures were placed in MINC ® Benchtop Incubators (Cook Medical Inc.) and only 2PN-derived embryos were collected. We sampled 20 μL of CM culture media per embryo in DNase and RNase-free sterile PCR tubes and froze them at −40°C. Unused culture medium was the negative control.

Free DNA in culture medium was purified using QIAamp Circulating Nucleic Acid kit (Qiagen, Hilden, Germany) as described by the manufacturer, mixed with 30 μL of elution buffer, then quantified using a NanoDrop 2000 spectrophotometer (Thermo Scientific Inc., Waltham, MA, USA).

We established a digital PCR detection system with reference to primers in the literature. We then optimized and amplified the D-loop region of the mitochondrial (NC_012920.1) gene, and β-actin (NG_007992.1) was the internal reference gene. Table 1 shows the primers and probes.
Table 1 Primer and Probe Sequences

We used a QX200 droplet dPCR system (Bio-Rad Laboratories Inc., Hercules, CA, USA). We found mtDNA in 223 samples, (Average: 219.80, n = 223). We did not detect gDNA in 25 samples (Average: 8.04, n = 198), and the minimum detection value was 1.8. Therefore, the number of copies of gDNA that were not detected were counted as 1.8.

The primers and probes in this paper were diluted to 10μM (10μmol/L). Reaction mixtures (20 μL) contained 10 μL ddPCR Supermix for Probes (No dUTP) (Bio-Rad Laboratories Inc.), forward and reverse primers (1.8 µL) for the target and β-actin genes, the target gene probe (0.4 µL), the β-actin gene probe (0.4 µL), and 2 µL of template.

Reaction mixtures (20 µL) were added to 8 holes in the middle row of a DG8 cartridge (20 μL X control buffer doubled for ~8 samples). About 70 µL of microdrop-generated (DG) oil was added to the bottom row of 8 holes in the cartridges, and all holes were filled. The gasket was covered and all holes on both sides were secured. The holder was placed above the QX200 microdroplet generator to produce microdroplets for 2 minutes in the top row of the cartridge. These were then transferred to 96-well plates, covered with a membrane and heat-sealed.




Nature Journal: Reflections on glittering 'mica' minerals

Two thousand years BC, when thin sheets were used as surfaces for painting mythological scenes, it was supposed that mica represented the preserved form of lightning flashes.

It is a generic term that applies to a group of complex minerals with a sheet or plate-like structure. All micas form flat six-sided crystals with cleavage parallel to the direction of the large surfaces, which permits them to be split into optically flat films.

Muscovite micas, which are colorless to pale green in color, are more easily split than others. The designation "muscovite" was derived from Muscovia, the district in Russia where the mineral was found and identified in the early seventeenth century. "Sheet" mica refers to books (or lenses) mined from either hard rock or loosely consolidated clayey materials. These can be readily split into specific thicknesses.

The Peabody Museum at Harvard has a beautiful serpent carved from trade mica. The surface is scored with shallow decorative incision marks in the location of the serpent's head. These perhaps signify the horned serpent figure similar to the Uktenas in Cherokee lore. The cut-out was assembled from a total of twelve mica fragments.

In other words, the Native Americans were meticulous when it came to crafting mica ornamentals. It was the material of choice for artwork throughout eastern North America for centuries — and the Cherokees had prospecting rights. .

Before mica mining became a multimillion dollar industrial enterprise in the area of Spruce Pine north of Asheville, it was a cottage industry. In The French Broad (1965), one of my favorite books, Wilma Dykeman, one of my favorite people, described those days:

"A large part of this mining is done in small operations — `groundhog holes,' the local people call them, penetrating the sides of hill after hill in these counties (Mitchell, Yancey and parts of Avery), and the raw wound of many an abandoned digging gapes on the mountainside, giving the country an appearance different from the rest of the French Broad watershed."

She also noted that it was during the post-Civil War era — "when a northern traveler happened to see a large sheet of `isinglass' in one of the cabins, where he stopped overnight" — that the sheet-mica business was initiated, thereby supplying "practically all of the isinglass used in the old-fashioned stove windows in this country or Canada." Big sheets of mica used for cabin or store windows were called isinglass. Smaller pieces were also crafted into kerosene lamp chimneys.

The center of mica production has traditionally been in the Spruce Pine area, but there were also extensive mines in Jackson and Macon counties. In "The History of Jackson Country" (1987), John L. Bell notes that mica deposits were first discovered "on the road between Webster and Franklin" in 1858. These were displayed at the South Carolina State Fair in 1866. As recently as the early 1940s, "the defense needs of World War II caused a boom in mica," then very "important in the production of electronic vacuum tubes. Ninety-four mines were opened in 1942, but "only 30 were operating in 1943 because of the manpower shortage."




Rosie Huntington-Whiteley turns heads in an aquamarine mini dress on date night with Jason

And Rosie Huntington-Whiteley, 34, ensured all eyes would be on her as she enjoyed a date night with fiancé Jason Statham, 53, at Nobu in Malibu on Wednesday.

Strutting into the venue in an eye-catching blue twinset, the Vogue cover girl was a vision of beauty on the outing.

Rosie opted for an aquamarine midi dress with a scooped neckline that showcased her cleavage and a cut out hem that put her tanned and toned pins on full display.

She teamed the garment with a cardigan in the same striking blue that complemented her golden complexion.

Boosting her 5′ 9″ model frame, the mother-of-one teetered along in a pair of peach barely-there heels and tucked a knitted white clutch bag under her arm.

Jason looked typically dapper as he donned a navy suit over a white T-shirt teamed with smart trainers.

The pair were enjoying some quality couple time as their three-year-old son Jack, did not join them for dinner.

From Publisher: Mail Online



Rebecca Lobie flaunts her assets in a pink latex dress amid OnlyFans rumours | Daily Mail Online

She's rumoured to be starting her own OnlyFans account soon, after gaining more than 100,000 followers on Instagram thanks to her raunchy photos.

And Rebecca Lobie did little to quell the speculation on Thursday as she flaunted her sensational figure in a skintight latex dress in her latest upload.

The 'hot niece' of late wildlife conservationist Steve Irwin showcased her ample cleavage and toned legs in the flirty pink number.

'My fav party dress': Steve Irwin's 'hot niece' Rebecca Lobie flaunted her sensational figure in a skintight latex dress on Thursday amid rumours she's starting an OnlyFans account

She styled her blonde hair straight, opted for a glamorous makeup palette, and boosted her height with a pair of perspex heels.

'My fav party dress. Can't wait to head to the goldy again soon!' wrote the Sunshine Coast-based mother of two, 33, referring to her upcoming trip to the Gold Coast.

Rebecca shared another snap on her Instagram Stories of herself modelling a personalised sleepwear set, with her name 'Bec' printed on the front.

Over the weekend, Rebecca left fans wondering whether she's set to monetise her racy photos by starting an OnlyFans account.

From Publisher: Mail Online



Paris Hilton wows in a semi-sheer white dress with puffy sleeves | Daily Mail Online

And Paris Hilton was sure to turn heads in a clip she shared via Instagram stories on Wednesday that saw her dance at home to Diana Ross and The Supremes.

The socialite, 40, looked sensational as she slipped into a semi-sheer white dress that had dramatic puffy sleeves.

Wow! Paris Hilton looked sensational as she slipped into a semi-sheer white dress with puffy sleeves while dancing at home in a stylish clip she shared via Instagram on Wednesday

Paris' ensemble had a low-cut scooped neckline and was semi-sheer to show off a glimpse of her cleavage while she twirled around a room in her house.

The outfit also had a tulle skirt that fell to the floor and which the reality star pulled up around and over her face.

Her golden locks were brushed into a sleek, straight style and she wore a light palette of make-up for the occasion.

Paris also made sure that all attention remained on her ensemble by keeping her accessories to a minimum and just wearing diamond stud earrings.

From Publisher: Mail Online