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The former professional golfer has gained an army of online followers despite stepping away from the sport, regularly posing for daring photographs and sharing those with her 3.1m fans.
While there are those who are not fond of her snaps, Paige reckons that they can longer have complaints after watching the NCAA outdoor track and field championships 3000m steeple chase in Oregon.
Collegiate athlete Garrett Marsing was the unfortunate bearer of a wardrobe malfunction in that meeting, with television images appearing to show his manhood outside of his shorts.
She said in response: "I just watched a dude run with his nut sack out for 3000m in the NCAA championship. I don't want anyone complaining about my cleavage anymore."
Before saying in response to a fan: "I'm sorry I was born with t***. I'm not going to hide them because your pea brain can't comprehend the fact a woman can be smart, strong, and sexy."
Speaking on her own Playing A Round podcast before that, Paige hit out at old-fashioned country clubs and the perception some have of female golfers.
She said: "I've literally been on country clubs where guys are using range-finders to look at me from two fairways over. And I've had people say, like, 'who let her on?'"
Adding: "They don't make you feel welcome. They are not going to fully allow people to be members and be accepted."
What makes the Delta covid-19 variant more infectious? | Tech Exec
After the virus binds to ACE2, the next step is to fuse with the cell, a process that begins when enzymes from the host cell cut the spike at two different sites, a process known as cleavage. This kick starts the fusion machinery. If binding is like the key fitting in the lock, cleavage is like the key turning the deadbolt. "Without cuts at both sites, the virus can’t get into cells," says Vineet Menachery, a virologist at The University of Texas Medical Branch.
One of the mutations present in Delta actually occurs in one of these cleavage sites, and a new study that has not yet been peer reviewed shows that this mutation does enhance cleavage. And Menachery, who was not involved in the study, says he has replicated those results in his lab. "So it’s a little bit easier for the virus to be activated," he says.
Whether that improves transmissibility isn't yet known, but it could. When scientists delete these cleavage sites, the virus becomes less transmissible and less pathogenic, Menachery says. So it stands to reason that changes that facilitate cleavage would increase transmissibility.
It's also possible that Delta's ability to evade the body's immune response helps fuel transmission. Immune evasion means more cells become infected and produce more virus, which then potentially makes it easier for person carrying that virus to infect someone else.
In any case, in the US and UK, just around 42% of the population is fully vaccinated. In India, where the virus surged fueled in part by the rapid spread of Delta, just 3.3% of the population has achieved full vaccination.
At the press briefing, Fauci urged those who have not been vaccinated to get their first shot and reminded those who are partially vaccinated not to skip their second dose. The Biden Administration hopes to have 70% of the population at least partially vaccinated by the Fourth of July. In the UK, Delta quickly replaced Alpha to become the dominant strain, and cases are now on the rise. "We cannot let that happen in the United States," Fauci said.
Origin unclear: The Hindu Editorial on the source of the coronavirus - The Hindu
Over 17 months after WHO first reported a cluster of cases of pneumonia of unknown cause in Wuhan, China, scientists are yet to determine with certainty how the SARS-CoV-2 virus emerged. Much like other viruses, SARS-CoV-2 too could have a natural origin or somehow escaped from the coronavirus research lab in Wuhan, the epicentre of the COVID-19 outbreak. With no hard scientific evidence available to confirm the lab leak hypothesis, there are some scientific leads that support a natural origin. If it is a zoonotic spillover, the virus could have either directly crossed over from bats to humans or through an intermediate host. But till date, neither the bat species that hosts the SARS-CoV-2 virus nor the intermediate host has been found. China's secrecy and delay in reporting the Wuhan outbreak and in finding the natural host or the intermediary have further fuelled the lab spillover hypothesis. Finding the host animal can be daunting. While the civet cat and dromedary camel were quickly identified to be the intermediate hosts of SARS and MERS, respectively, it took years to identify the horseshoe bat that harbours SARS virus strains. To date, a complete Ebola virus has never been isolated from an animal source.
If the virus had been bioengineered, the genome sequence would carry tell-tale signs. But scientists have not found any signature of genetic manipulation. While a particular site (furin cleavage) on the SARS-CoV-2 spike protein that allows the virus to infect the cells has been cited as evidence of bioengineering, the fact is that it is not unique to SARS-CoV-2. A combination of nucleotides in the furin cleavage site that encode for a particular amino acid — another feature that is forwarded as supporting laboratory manipulation — too has been shown to be not unique. For instance, the nucleotide combination encoding for the amino acid is present in other sites of the SARS-CoV-2 virus and in the 2003 SARS virus. The possibility of SARS-CoV-2 evolving via cell culture appears bleak as scientists have found the virus losing features key to transmission and virulence unless cultured using new methods. Reports of three Wuhan lab researchers falling ill in November 2019 by itself does not prove a lab leak hypothesis. There is no evidence that they were infected with the SARS-CoV-2 virus, and even if they were, it is necessary to prove that it happened from inside the lab. In the absence of conclusive evidence to support either hypothesis so far, a thorough investigation is needed. While the inquiry by the U.S. intelligence might provide clues, a scientific investigation is more likely to help reach closure; China's cooperation, therefore, becomes vital and politicising the virus origin is not going to help.
Printable version | Jun 14, 2021 9:35:38 PM | https://www.thehindu.com/opinion/editorial/origin-unclear-the-hindu-editorial-on-the-source-of-the-coronavirus/article34807109.ece
From the abrogation of the special status of Jammu and Kashmir, to the landmark Ayodhya verdict, 2019 proved to be an eventful year.
Helen Hunt strikes a pose with a VERY busty Jasmine Cephas Jones at the premiere of Blindspotting
But Helen Hunt was supporting another sequel on Sunday when she hit the red carpet for the new Starz dramedy Blindspotting, a continuation of the critically acclaimed film of the same name.
Joining the 57-year-old Oscar winner was the series' lead actress, Emmy winner Jasmine Cephas Jones, 31.
Eye-catching: Helen Hunt, 57, looked classy in a strappy black top as she led the Los Angeles premiere of Jasmine Cephas Jones' new Starz series Blindspotting
Helen showed off a reserved yet stylish look built around a black sleeveless top with thick shoulder straps tied to a ring over her cleavage.
The 5ft7in Mad About You star elevated her stature with black suede platform boots, and she added some extra sparkle to her outfit with gold earrings decked out with dangling beads.
Brightening up: Helen contrasted the top with a mustard yellow set of slacks with a hypnotizing pattern, as well as black platform boots
Stunner: Blindspotting star Jasmine Cephas Jones sizzled on the red carpet thanks to her busty purple satin gown, which featured thick robe-like sleeves
Racy: The low-cut item highlighted her cleavage and was cinched around her waist with ruched fabric to emphasize her hourglass figure
Legs for days: The Hamilton star kicked a leg out thanks to her dress' high slit and revealed her black open-toe heels, which strapped all the way up her legs
Lab leak or natural? How the evidence stacks up in the coronavirus origin investigation
New Delhi: Over the last few weeks, voices that believe — and proffer scientific evidence in favour of — the hypothesis that the SARS-CoV2 virus leaked from a Chinese lab have grown stronger.
There have been a series of factors that have elevated the lab leak theory from conspiratorial speculation to an increasingly plausible possibility. These include China's reluctance to give foreign investigators full access to its laboratories and databases, and the seeming hesitation of the World Health Organization (WHO) to push the envelope with the Asian nation — refer to Republican Senator John Kennedy's questioning of Dr Anthony Fauci, the chief medical adviser to US President Joe Biden, during a Senate hearing last month.
Questioning the immunologist on the nature of research being conducted by the Wuhan Institute of Virology (WIV) on coronaviruses, Kennedy asked whether the WHO would fall out of Xi Jinping's pocket if the Chinese President were to be held upside down.
But no matter how compelling the evidence may seem, questions about the origins of the SARS-CoV-2 virus are by no means closed. There are theories and evidence, but there is also the other side — counter-arguments.
The scientists said a "Master's thesis (in the Chinese language) was found on the cnki.net website which described in detail the severe illness in miners".
"The thesis concluded that a SARS-like CoV originating from Chinese horseshoe bats (Rhinolophus) was the predicted causative agent. The cases were remotely monitored by a prominent pulmonologist in China. Retrospective analysis of the pneumonia cases shows striking similarities with Covid-19," they added.
Victoria Beckham's new sizzling snap will take your breath away
Victoria Beckham dropped jaws as she flaunted her ageless beauty in new Instagram photo, looking drop-dead gorgeous in the sizzling outfit.
The David Beckham's sweetheart, undoubtedly, looked out of this world as she showed off her elegance in the figure-hugging blue dress while promoting the new additions to her Victoria Beckham beauty range.
The renowned fashion designer gave fans major style envy in her latest Instagram upload. Her gorgeous dress featured two racy cut-out sections – one across the cleavage and one peephole positioned across her waist, showing off her taut abs.
In the photo, the former Spice Girls member is seen adding a slick of the amour lip tint from her beauty line to her plump pout.
The 47-year old's brunette-coloured locks were styled into on-trend mermaid waves, elevating her beauty.
Victoria Beckham's fans were quick to respond to her amazing snap, showering praise on the fashion sensation's ravishing look.
90 Day Fiancé: Annie Suwan Wows In New Shocking Hairstyle & Color
90 Day Fiancé fans are loving Annie Suwan's new hairstyle and color, with David Toborowsky's wife flaunting curly hair with blonde highlights. Recently, 90 Day Fiancé celeb Jenny Slatten also ditched her honey blonde hair and tried a few shocking new hair colors. She didn't actually dye her hair but tried different shades of red, pink and teal on her picture with the help of an app. Many fans loved Jenny with pink and red hair. However, most viewers preferred her original hair color.
After massive weight loss, Angela Deem is transforming her style as well. She was recently spotted with pink hair. The 55-year-old meemaw also showed off her new glamorous style while recording a Cameo for a fan. She wore stunning makeup and tied her bleached hair in a slicked-back ponytail. Angela wore a gray tank top and flaunted her bigger cleavage. Earlier, fans were worried that she had started looking like an older woman due to sagging skin. But the 90 Day Fiancé star's new pictures suggest that she has probably had a facelift, which is making her face look more attractive.
It seems like Pillow Talker Annie also got inspired to experiment with her hair. She recently posted a close-up of her face with a new hairstyle and color. The Thailand native sported curly hair with blonde streaks and captioned the post, " My Hair style looks Cute in this picture. " In the picture, Annie is wearing oversized sunglasses and her wedding ring. The 28-year-old beauty's natural hair color is black, and she has straight hair. However, she often likes to color her hair blonde. Check out the picture Annie posted on Instagram:
90 Day Fiancé star Darcey Silva , who is popular for posting unrealistic pictures, loved Annie's new hairstyle. Many fans complimented her new style and wrote, " Pretty love the hair, " " I like the curls, " " It's beautiful and the color looks good on you " and " You're always cute but I like it darker. " Her husband David commented, " You are beautiful in every way. " David and Annie are the 90 Day Fiancé franchise's most popular couple. Even though the couple has a 24-year age gap, they are still madly in love with each other.
They can often be spotted going on dates and enjoying luxurious vacations together. A few weeks back, the duo enjoyed some quality at the W Hotel in Scottsdale and posted a couple of cozy pictures with the hashtag #spicyitupwhitdavidandannie. 90 Day Fiancé 's David and Annie also gave boom boom tips to other fans to help them in the bedroom. David told husbands that their wives will smile from ear to ear if they follow his secret bedroom tips. He may have some experience because fans can see his 90 Day Fiancé co-star and wife Annie always giving a big smile to the camera.
Neha Nathani is a Reality TV writer at ScreenRant, and she loves it! She has always been passionate about telling and writing stories. But when she isn't writing, she plays with her cats or tries some new dance moves.
Polarized endosome dynamics engage cytoplasmic Par-3 that recruits dynein during asymmetric cell
In the developing embryos, the cortical polarity regulator Par-3 is critical for establishing Notch signaling asymmetry between daughter cells during asymmetric cell division (ACD). How cortically localized Par-3 establishes asymmetric Notch activity in the nucleus is not understood. Here, using in vivo time-lapse imaging of mitotic radial glia progenitors in the developing zebrafish forebrain, we uncover that during horizontal ACD along the anteroposterior embryonic axis, endosomes containing the Notch ligand DeltaD (Dld) move toward the cleavage plane and preferentially segregate into the posterior (subsequently basal) Notch hi daughter. This asymmetric segregation requires the activity of Par-3 and dynein motor complex. Using label retention expansion microscopy, we further detect Par-3 in the cytosol colocalizing the dynein light intermediate chain 1 (Dlic1) onto Dld endosomes. Par-3, Dlic1, and Dld are associated in protein complexes in vivo. Our data reveal an unanticipated mechanism by which cytoplasmic Par-3 directly polarizes Notch signaling components during ACD.
As shown previously, in the developing zebrafish forebrain, most of the RGPs undergo ACD to generate an apical differentiating daughter with low Notch activity and a basal self-renewing daughter with high Notch activity ( 20 ). To understand how such Notch signaling asymmetry arises, we visualized internalized Notch ligand Dld using an antibody uptake assay ( 34 ) and in vivo time-lapse imaging. Intriguingly, only punctate cytoplasmic labeling was observed ( Fig. 1A ); no Dld was accumulated on the plasma membrane of mitotic RGPs. In the mib mutant, which disrupts a conserved ubiquitin E3 ligase essential for Notch ligand endocytosis ( 35 ), the punctate cytoplasmic labeling of Dld was, however, lost; fluorescence was instead largely concentrated on the plasma membrane (fig. S1A). These data indicate that the punctately labeled structures are internalized Dld in endosomes (in short, Dld endosomes). They also suggest that, in mitotic RGPs, Dld undergoes active endocytosis to be predominantly distributed in endocytic vesicles. We also evaluated whether this labeling method affected embryonic development or RGP cell division modes. The developing forebrain RGPs at this developmental stage predominantly undergo horizontal division (with the division axis parallel to the ventricular surface). No substantial differences in embryonic morphology and RGP division modes were observed between control and Dld antibody-injected embryos (fig. S1, B to D).
We next performed automated tracking analysis of 19 RGPs, which were captured throughout their entire mitotic cell cycle (from prophase to telophase) and, moreover, with consistent tracking of Dld endosomes in all frames. Tracking of more than 300 Dld endosomes from all 19 cells throughout the RGP mitotic cycle (45 time points) allowed us to visualize the progressive enrichment of endosomes toward the posterior daughter [ Fig. 1E (E1) and movie S2]. Such enrichment could be due to directional endosome movement toward the posterior, their selective degradation at the anterior, or both. The presence of supernumerary-labeled Dld endosomes made it challenging to unambiguously discern individual endosome's trajectories. Intriguingly, because of the mosaic nature of our Dld-labeling method, some RGPs contained only a single labeled Dld endosome. This enabled us to clearly track the movement of individual endosomes. We observed that the Dld endosome first moved toward the cleavage plane, followed by a directed maneuver toward the posterior side [ Fig. 1E (E2 and E3) and movies S3 to S6]. Together, these data uncover polarized dynamics of Dld endosomes during horizontal RGP division and show that Dld endosomes are asymmetrically segregated into the posterior daughter in most of the RGP divisions.
( A ) Schematic depicting the asymmetry of Dld endosomes (Dld hi ) and Notch activity (N hi ) in daughter cells. ( B ) Topology and statistics of relative daughter cell position along A-P (anteroposterior) and Ap-Ba (apicobasal) axes after horizontal division. MIP of 5-μm z-stacks (1-μm z-step) is shown. ( C ) Time-lapse images showing that Dld endosomes and Mib are segregated into different daughter cells following RGP division ( n = 25). MIP of 5-μm z-stacks (1-μm z-step) is shown. The time interval between z-stacks is 20 s, and the total acquisition time is 30 min. ( D ) The top left graph plots individual RGP's asymmetry indices for Mib-GFP ( x axis) and internalized Dld ( y axis). The top right graph shows the distribution of asymmetry indices for Mib-GFP and Dld endosomes; the dotted lines indicate the threshold of |0.2| for calling asymmetry. *** P < 0.0001, t = 6.549, df = 48, n = 25; unpaired two tailed t test. Mean with SEM is shown. The bottom pie chart shows the percentage of RGPs with indicated distribution patterns. n = 25 RGPs, from eight embryos of five repeat experiments. ( E ) Time-lapse images of a clonally labeled RGP (green) showing preferential segregation of internalized Dld to her4.1-dRFP hi daughter. MIP of 8-μm z-stacks (1-μm z-step) is shown. The time interval between z-stacks is 6 min, and the total acquisition time is ~10 hours. ( F ) Plot for quantifying her4.1 -dRFP and internalized Dld in daughter RGPs 1 hour after anaphase. n = 8 RGPs, from eight embryos of six repeat experiments.
Because of the lack of an anti-Notch antibody that works in zebrafish, we took three different approaches to address this question. First, most RGPs that we imaged in the developing zebrafish neurogenic forebrain undergo horizontal divisions along the A-P axis (fig. S1, C and D). Shortly after division, daughter cells begin interkinetic nuclear migration and adopt differential position along the apicobasal (Ap-Ba) axis. The basal daughter is previously shown to be Notch hi ( Fig. 2B , left) ( 20 ). We therefore determined the relationship between the A-P daughter position immediately following RGP mitosis and the Ap-Ba daughter position shortly thereafter. Among 42 pairs of daughter cells with observable differences in their position along the Ap-Ba axis, most (67%) had more Dld in the posterior daughter that initiated an earlier basal migration ( Fig. 2B , right). These results suggest that Dld endosomes are preferentially segregated to the posterior daughter, which later becomes the basal Notch hi daughter.
The second approach to discern the relationship between Dld endosome segregation and Notch activity involved analysis of the E3 ubiquitin ligase Mib, which is asymmetrically segregated to the apical-differentiating daughter following RGP ACD in zebrafish ( 20 ). Similar observations are also reported in the chick neural progenitors ( 37 ). By simultaneously tracking Dld endosomes and Mib distribution in 25 RGPs, we found that they were largely segregated into different daughter cells ( Fig. 2C , fig. S4, and movie S7): 64% RGPs had anteriorly enriched Mib while posteriorly enriched Dld ( Fig. 2D ). Note that Mib–green fluorescent protein (GFP) showed anterior enrichment early on during the cell cycle, when Dld distribution appeared random. While this observation implied a potential asymmetric endocytosis, it does not appear to contribute to Dld asymmetry as internalized Dld appeared randomly distributed in RGPs during prometaphase. Together, this observation supports the notion that Dld endosomes preferentially segregate to the posterior-then-basal Notch hi daughter and away from the Mib-high apical daughter.
Imaging of internalized Dld in par-3 morphants uncovered that, while Dld endosomes underwent largely normal subcortical association and congregation toward the future cleavage plane, their final asymmetric segregation into the posterior daughter was significantly disrupted ( Fig. 3, B and E to G , and movie S9). This defect was rescuable by Par-3-GFP mRNA injection (fig. S6). These data, together with the observed knockdown of Par-3 protein in RGPs (fig. S8), validate the MO specificity and efficacy in our system and suggest that Par-3 is essential for polarized segregation of Notch signaling endosomes during RGP ACD.
( A to D ) Time-lapse sequence of images showing Dld endosome dynamics in mitotic RGPs from 28-hpf control MO (A) and embryos deficient for par-3 activity (B), treated with the dynein inhibitor CBD (C), deficient for dynein intermediate light chain 1 ( dlic1 ) (D). Centrosomes are labeled with GFP-centrin, and membrane is labeled with Myr-TdTomato reporter. For (A) to (D), all images shown are the MIP of five confocal z-stacks (1−μm z-step). The time interval between each volume of z-stacks is 15 s, and the total acquisition time is 25 min. ( E ) Kymograph of horizontal projection of (A) to (D) showing distribution of all tracked Dld endosomes along the anteroposterior (A-P) axis ( x ) over time ( y ). The red line delineates center point of the axis defined by two centrosomes. ( F ) Scatter plot showing asymmetry indices in telophase RGPs. Thirty-three control MO RGPs were from 25 embryos of eight repeat experiments, 22 par-3 MO RGPs were from 9 embryos of six repeat experiments, 15 CBD-treated RGPs were from 7 embryos of four repeat experiments, and 15 dlic1 MO RGPs were from 8 embryos of four repeat experiments. The unpaired two tailed t test shows significance between ctrl versus par-3 MO, **** P < 0.0001 ( t = 4.706, df = 53); ctrl MO versus CBD, * P = 0.0129 ( t = 2.589, df = 46); ctrl versus dlic1 MO ** P = 0.0007 ( t = 3.645, df = 46). Mean with SEM is shown for each group. ( G ) Bar graph showing the percentage of RGPs with different patterns of internalized Dld distribution. Disruption of either Par-3 or dynein activity results in a significant decrease of asymmetric posterior Dld segregation. **** P < 0.0001, χ 2 test (chi-square = 95.62, df = 6).
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