Nicki Minaj is back with a bang and already making waves with her surprise re-release of her 2009 mixtape "Beam Me Up Scotty" on streaming platforms , featuring artists like Drake, Lil Wayne, Gucci Mane and more.
Over the past few days the 38-year-old rapper has been posting racy, almost nude, pictures on her Instagram account.
In her most recent post the "Super Bass" rapper was fully dressed but didn't shy away from showing off her cleavage.
Accessorising the look with layers of chunky silver necklaces and which included her jewelled signature Barbie neckpiece , bold silver earrings as well as a purple wristwatch.
She switch her look from yesterdays super long black wig to long blonde hair with light pink roots.
Last Friday the star crashed the Crocs website after posting a wearing nothing but a pair of bright pink Crocs with dazzling silver details.
We Can't Believe Dua Lipa Showed THIS Much Cleavage On TV–Her Brits Awards Dress Is Too Hot For
Dua Lipa cleaned up at the Brit Awards on Sunday, including taking home the award for British Female Solo Artist and British Album of the Year, but it was her leggy custom Vivienne Westwood red carpet look that our editors are still buzzing about.
The “Don’t Start Now” singer arrived at the awards show in London wearing the jaw-dropping outfit styled by collaborator Lorenzo Posocco . Her legs look incredible!
The “Future Nostalgia” winner, who also performed in a Union Jack-printed outfit during the show, looked flawless in the yellow off-the-shoulder mini dress complete with a train. Oh, and don’t forget about the black garter stockings she wore to complete the retro look!
Our jaws are still on the floor after seeing what the 25-year-old singer wore to the UK’s equivalent to the Grammys . Westwood is known for sexy corset tops and dresses, but Dua’s look takes it to a new level.
Of her choice to wear the “British punk fashion brand,” Dua told reporters on the red carpet that night, “I wanted to make it British, quite London, have some fun with it.” Well, we think she achieved that!
Makeup artist Lisa Eldridge completed Dua’s “Rococo Punk Princess” vibes with strong brows, blush and a glittery eye. Flawless!
A weak COPI binding motif in the cytoplasmic tail of SARS-CoV-2 spike glycoprotein is necessary
The SARS-CoV-2 spike glycoprotein (spike) mediates viral entry by binding ACE2 receptors on host cell surfaces. Spike glycan processing and cleavage, which occur in the Golgi network, are important for fusion at the plasma membrane, promoting both virion infectivity and cell-to-cell viral spreading. We show that a KxHxx motif in the cytosolic tail of spike weakly binds the COPβ’ subunit of COPI coatomer, which facilitates some recycling of spike within the Golgi, while releasing the remainder to the cell surface. Although histidine (KxHxx) has been proposed to be equivalent to lysine within di-lysine endoplasmic reticulum (ER) retrieval sequences, we show that histidine-to-lysine substitution (KxKxx) retains spike at the ER, and prevents glycan processing, protease cleavage, and transport to the plasma membrane.
Supplementary Materials | Science
Tao Yue, Xiaoming Zhan, Duanwu Zhang, Ruchi Jain, Kuan-wen Wang, Jin Huk Choi, Takuma Misawa, Lijing Su, Jiexia Quan, Sara Hildebrand, Darui Xu, Xiaohong Li, Emre Turer, Lei Sun, Eva Marie Y. Moresco, Bruce Beutler
Lily James is 'loves wearing fake DD breasts on AND off set while playing Pamela Anderson' |
Lily James reportedly loves wearing a fake 34DD bust 'on and off' the set of Pam & Tommy because it helps her get into character.
The actress, 32, is playing Baywatch icon Pamela Anderson in the upcoming biopic and has been seen sporting a large cleavage at the beach in recent days.
And now a source has told The Sun On Sunday that the star, who has a 'modest' natural B cup, is 'having a blast' playing 'dress up with the chest piece'.
'She's having a blast': Lily James reportedly loves wearing a fake 34DD bust on and off the set of Pam & Tommy as it helps her get into character (pictured filming Baywatch scenes)
Speaking with the publication, a pal said: 'Lily's having a blast getting into character and playing dress up with the fake chest piece.
'The boobs really help her get into character and she loves walking around in them, especially as naturally she has a more modest bust.'
Lily is the spitting image of Pamela, now 53, in snaps taken during filming and is thought to be wearing prosthetics designed to emulate Pamela's famously bust.
Busty: A source has claimed that star, who has a 'modest' natural B cup (right in 2019) , is 'having a blast' playing 'dress up with the chest piece' (left, as Pamela on Thursday)
Pamela's cleavage is said to be the result of two breast implant surgeries in the early 1990s, increasing her breast size to a 34D, and then to a 34DD respectively.
Andy Cohen Denies Erika Jayne is Refusing to Appear on WWHL Without Pre-Selected Questions as
After a rumor began swirling on social media which suggested the Real Housewives of Beverly Hills cast member was attempting to prevent any uncomfortable moments from arising, host, Andy Cohen sets the record straight.
On May 14, the Bold and the Bravo on Instagram claimed Erika has “alerted the executives at Bravo that she will not be appearing on WWHL unless she has pre-selected questions.”
According to the report, Erika was doing so “in an effort to preserve her already shattered image,” and to prevent fans from calling in with questions on the night of her episode.
While it doesn’t seem that outlandish to think that Erika would want to avoid being put on the spot regarding her ongoing divorce from Thomas Girardi and the many lawsuits against them, Andy has shot down the Bravo fan page’s claims.
“Yeah this isn’t true,” he wrote in a message, via Comments by Bravo on Instagram .
In other Erika Jayne news, the longtime RHOBH cast member was spotted out in Los Angeles on Thursday, where she was filming a segment for the upcoming 2021 MTV Movie and TV Awards .
In photos, Erika was seen donning a blue latex dress and matching coat, which she paired with layered silver and gold cross necklaces.
Erika Jayne dazzles in a bright blue latex dress and matching coat https://t.co/VdzAhCXesS via @DailyMailCeleb
Love Island's Demi Jones showcases her toned frame in a colourful halterneck dress | Daily Mail
And Demi Jones continued her recovery as she stepped out in London for a glamorous night out on Saturday.
The Love Island 2020 star, 22, displayed her cleavage and toned legs in a multicoloured halterneck dress as she headed to a rooftop bar in the capital.
The vibrant purple and orange gown was paired with strappy orange sandals and a nude handbag as she walked along.
Her flame red tresses were pulled into a sleek ponytail while a glamorous palette of make-up enhanced Demi's stunning features.
She took to Instagram to share a post-surgery selfie as she explained she was 'very sleepy' and that the procedure was 'painful'.
After the revelation she thanked fans and said she was 'overwhelmed with support' and said she had received 'thousands of kind messages'.
Posting a gorgeous snap of herself sporting a full face of glam and wearing an off-white sweatshirt, the reality star penned: 'I'm so overwhelmed with support.'
The structure of an infectious immature flavivirus redefines viral architecture and maturation |
In this work, we show that the immature form predominates the viral preparations of BinJV and, unlike for VIFs, is infectious without the requirement of additional antibody-mediated uptake. Antibody-mediated inhibition of maturation neutralizes BinJV but is isotype dependent. The homogeneity of the BinJV particles allowed for the first structure determination of an immature flavivirus with an unambiguous topology that is likely to be conserved across all flaviviruses. The unexpected architecture supports a model of maturation where a centrally placed prM activates and guides a collapse of the spike.
To investigate the structure of an ISF, we purified BinJV from infected C6/36 cells for cryo-EM imaging. SDS–polyacrylamide gel electrophoresis (PAGE) analysis of the preparation revealed that it was mostly immature, with a prominent band for prM and only traces of mature M ( Fig. 1A ). Similar levels of prM were observed over multiple infection and purification rounds and were also observed for two other ISFs, Hidden Valley virus (HVV) and Parramatta River virus (PaRV), suggesting a conserved ISF feature ( Fig. 1A ). For comparison, a preparation of the VIF WNV kunjin subtype (WNV KUN ) is also shown ( Fig. 1A ). Supernatant titers of BinJV are similar to WNV KUN , suggesting that viral release is likely efficient and not a major contributing factor to explain the predominance of uncleaved prM in BinJV preparations.
( A ) SDS-PAGE analysis of gradient purified BinJV, HVV, PaRV, and WNV KUN virions. Virus preparations are nonreduced and stained with SYPRO Ruby stain. E, prM, capsid (C), and M proteins indicated by arrows. ( B ) Surface cryo-EM reconstruction of BinJV. Density attributed to the pr proteins within the immature form is shown in yellow, and the three E proteins that make up the asymmetric unit were shown in shades of green. Ellipses, triangles, and pentagons represent the two-, three-, and fivefold icosahedral axes, respectively. ( C ) The asymmetric unit of BinJV is composed of three copies of E (green, pink, and cyan) and prM (dark green, magenta, and blue). ( D ) Side view of the asymmetric unit displayed as a cartoon and colored by symmetry position. Density is shown for prM. Lines illustrate the extent of the lipid bilayer.
Despite variations upstream from the furin cleavage site, the R88-D64 interaction between the furin site and the neighboring prM is also present in other lineage II ISFs, DENV-4, and possibly other DENV serotypes that have a histidine residue at the equivalent of BinJV position 88. This additional salt bridge could potentially explain the predominance of uncleaved prM in purified BinJV and other viruses sharing this feature. At the sequence level, the cleavage site of BinJV (R-S-R-S-K-R) appears compatible with the recognition site of the proprotein convertase, furin (R-X-R/K-R), suggesting that cleavage should occur if the site is accessible. To empirically assess the capacity for processing, we subjected purified BinJV particles to in vitro furin cleavage at a range of pH conditions (pH 5.0 to 8.0). An immature WNV KUN particle preparation produced using the addition of NH 4 Cl was included as a VIF control. As determined by SDS-PAGE, cleavage occurred between pH 5.5 and 6.5 for BinJV and pH 5.0 and 6.0 for WNV KUN ( Fig. 3A ). This is in agreement with previous studies, which have demonstrated that cleavage is dependent on the low pH of the secretory pathway ( 18 – 20 ) and suggest that, like VIFs, BinJV immature virions undergo acid-triggered conformational rearrangements that facilitate furin-like protease processing of prM.
( A ) Purified BinJV (left) and immature WNV KUN (right) were incubated with furin in buffer ranging from pH 5 to 8 and analyzed by nonreducing SDS-PAGE and visualized by SYPRO Ruby stain. Viral proteins indicated by arrows. ( B ) Infectivity of BinJV and immature WNV KUN virions in C6/36 cells with or without furin treatment. Cleavage was analyzed by SDS-PAGE, and virus titers were determined by TCID 50 . n = 3 biological replicates, and statistical analysis was performed by two-stage multiple t tests against nontreated (furin negative). * P ≤ 0.05 and ** P ≤ 0.01. Means ± SD. Limit of detection (L.O.D.) for TCID 50 is 2.3 log 10 TCID 50 ml −1 . ( C ) Infectivity of BinJV, PaRV, and WNV KUN in C6/36 cells pretreated with or without 25 μM furin inhibitor (FI). Virus titers were determined by TCID 50 ; n = 3 biological replicates, and statistical analysis was performed by two-stage multiple t tests against nontreated (FI negative). * P ≤ 0.05 and *** P ≤ 0.001. Means ± SD. Limit of detection for TCID 50 is 2.3 log 10 TCID 50 ml −1 .
Earlier studies have shown that in vitro maturation by furin of immature VIFs significantly increases infectivity ( 9 , 20 , 21 ). We tested the impact of cleavage on the infectivity of BinJV particles by infecting C6/36 cells and assessing the output by median tissue culture infective dose (TCID 50 ). We observed that both BinJV and WNV KUN had an approximately 10-fold increase in infectivity when the virus was matured by furin ( Fig. 3B ). These results confirm that, despite its predominantly immature phenotype, the cleavage of prM enhances BinJV infectivity.
Most of the intraspike interactions involve prM with only one direct contact interface between E molecules. In the absence of these pr-mediated interactions, the spike is not predicted to be stable with only one remaining interface burying just 146 Å 2 (fig. S5). Thus, a collapse of the spike does not require a pull force. The role of prM during maturation may instead guide the collapse toward the underlying M, which undergoes little structural change ( Fig. 4D and movie S1).
A strictly conserved histidine residue, H243 in ED-II of the E protein, is in close proximity to the prM-prM salt bridges between R88/R90 and D64 ( Fig. 2D ). Protonation of this residue during egress introduces a buried positive charge that may destabilize the prM-prM dimeric interface and release the furin site, acting as a pH switch to facilitate the maturation process.
How the collapse results in a transition from 60 trimeric spikes to 90 head-to-tail dimers ( Fig. 4D and movie S1) may be explained by key elements in the BinJV structure. First, we observe that, as in immature particles, the spike is present as an asymmetric trimer. Departure from a low-energy, symmetrical trimer results from a ~55° twist of the pr/E components of prM-E3 ( Fig. 4E ). This arrangement is locked in place by a large dimeric interface between the pr domains of prM2 and prM3 at the top of the spike ( Fig. 4C ). By contrast, the membrane-anchored M domains follow a quasi-threefold symmetry.
Tech manager who called Bay Area women 'soft and weak' fired
A former Facebook product manager says Apple fired him shortly after hiring him following an employee protest about allegedly sexist and racist language in his best-selling book Chaos Monkeys.
Apple earlier told Reuters in response to questions about his departure that, “Behavior that demeans or discriminates against people for who they are has no place here.”
García-Martínez said on Twitter that the company’s statement implied he’d engaged in “negative behavior” while at Apple. “That is defamatory and categorically false,” he tweeted . He did not directly address the allegations by Apple employees about his book. He did not immediately respond to a request for comment from this news organization about those claims.
García-Martínez’ exit followed news from technology website The Verge on Wednesday that more than 2,000 Apple employees had signed a petition expressing deep concern about his hiring. The letter, according to the site, focused on García-Martínez’ 2016 autobiography, and cited a number of statements from it.
“Most women in the Bay Area are soft and weak, cosseted and naive despite their claims of worldliness,” one passage said, contrasting what the author believed of the region’s women against what he saw in a girlfriend who in a “post-apocalypse” would be capable of “carpentry, animal husbandry, or a shotgun blast to someone's back.”
On Friday afternoon, García-Martínez said Apple had been “well aware” of his writing before bringing him on. “My references were questioned extensively about my bestselling book and my real professional persona (rather than literary one),” he tweeted.
The petition cited another passage about a different woman, of whom he wrote: “Unlike most women at Facebook (or in the Bay Area, really) she knew how to dress; forties-style, form-fitting dresses from neck to knee were her mainstay.” He also complained that few Facebook employees put their “femininity on display in the form of dresses and heels.” Elsewhere, he described a woman in a meeting at Facebook as needing “more cleavage or more charm.”
The Apple employees accused García-Martínez of both sexism and racism. They cited another passage referring to an engineering manager with a “thick Indian accent” who reminded him of an “auto-rickshaw driver” in India.
García-Martínez said on Twitter that he had until Friday afternoon kept silent about the matter because he wanted to “settle things amicably with the company that I admired, and at which I hoped to build the future of ads privacy.” He said he was speaking out because Apple had spoken publicly about his departure.
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