Monday, April 26, 2021

Combined RT-qPCR and pyrosequencing of a Spike glycoprotein polybasic cleavage motif can uncover

Combined RT-qPCR and pyrosequencing of a Spike glycoprotein polybasic cleavage motif can uncover

CONCLUSIONS: The proposed protocol allows a specific and sensitive confirmation of SARS-CoV-2 infections close to the detection limits of RT-qPCR. The tested biotinylated primers do not negatively affect the RT-qPCR pipeline and thus can be optionally applied to enable deeper inspection of RT-qPCR results by subsequent pyrosequencing. Moreover, due to the incremental transmission of SARS-CoV-2 variants of concern, we note that the used strategy can uncover (Spike) P681H allowing the pre-selection of SARS-CoV-2 B.1.1.7 candidate specimens for deep sequencing.

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From Publisher: Docwire News



Carol Vorderman, 60, flashes cleavage in string bikini as she pays tribute to Wales - Daily Star

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The former Countdown bombshell, who celebrated her milestone 60th birthday at Christmas, couldn't resist sharing a steamy snap from her archives to show off her ageless figure.

In the busty pic, Carol can be see sizzling in a racy bikini top that includes the signature Welsh dragon from the national flag.

The striking beachwear showcases the Welsh symbol splattered across the stretch material used to conceal her dignity.

Oozing sass, Carol posed up a storm in the mirror as she captured the sexy image herself using her smart phone.

Ramping up the sex appeal, her blonde tresses were slightly roughed up but glamorously styled poker straight.

Carol had been busy enjoying a trip to her hometown in Wales this weekend and her Instagram uploads have certainly been popular among her legion of fans.

The self-confessed walk-a-holic set out on a mission to find some great treks while wearing figure hugging animal print leggings.

During a lengthy beach walk, Carol couldn't resist taking to the sea but explained she'd got carried away in her welly's and ended up "soaked".

From Publisher: Dailystar.co.uk



Oscars fashion trends: midriffs and statement tuxedos catch up with Gen Z style | Fashion | The

For Generation Z, a plunging neckline is what mums wear in date-night photos they post on Facebook. A crop top above high waisted jeans, to show a few inches of skin, or a dress with a shark-bite sized cut out to show some side-ab, is a more modern way to power (party) dress.

Carey Mulligan's gold sequins showcased two ribs between a vast skirt and a tiny boob tube. (Mulligan chose to leave the matching face mask with which the look was accessorised when it starred on the Valentino haute couture catwalk in January at home.) Andra Day, fellow best actress nominee for her portrayal of Billie Holiday, also wore gold sequins with an emphasis on abdomen. "There's cut-outs, leg, peekaboo – it's a scandal," Day's stylist Wouri Vice told Vogue. The dress was inspired by Bob Mackie's daring 1980s Oscar gowns for Cher, created by Vera Wang – and engineered with the aid of a welder. Zendaya's sunshine yellow Valentino was bikini on top, ballgown below; Vanessa Kirby's pale pink Gucci had a delicate half moon gap above the waistband.

In an era when Hollywood fortunes are made by high-kicking roles in all-action blockbusters, rather than by nailing the America's sweetheart role, perhaps it makes sense that dresses that show off boxer abs are edging out the meringue-adjacent, prom-princess aesthetic. As far as body positivity in popular culture goes, however, the power side-ab is at best a sideways move.

Time was that red carpet fashion was about women, with menswear relegated to a final paragraph referencing the odd novelty bow tie. Not any more. Minari director Lee Isaac Chung commissioned the Asian American brand Goodfight to make his shawl-collar tux with pocket-chain detailing, which he teamed with yellow-stitched Dr Martens; his nine-year-old star, Alan Kim, was dressed in tailored shorts by menswear supremo Thom Browne. Colman Domingo of Ma Rainey's Black Bottom wore a fuchsia three-piece Versace suit with a matching shirt, while his co-star Daniel Kaluuya wore a double-breasted Bottega Veneta tuxedo with a necklace of Cartier diamonds so large and bright they looked like a string of pearls. Leslie Odom Jr wore a gold shirt to match his gold Brioni suit.

The aesthetic of Nomadland, which dominated the night, is sumptuous to look at while rigorously anti-glamour. The film is bathed in natural light, finding beauty in rugged landscapes and bare faces. Fittingly, director Chloé Zhao collected her Oscar wearing white sneakers with her understated oyster-toned Hermes dress, and wore her hair in plaits rather than a Hollywood blow-dry; Frances McDormand wore long sleeved black, stark and simple but for the feathers at the cuffs.

From Publisher: the Guardian



Oscars: All About Ann Roth, Now the Oldest Oscar Winner at Age 89, and The Fashion of the Academy

But, had she attended, what would Ann Roth have said or worn collecting her umpteenth Oscar for costume design? A second-time winner at 89, like Anthony Hopkins at 83, she sat this one out. In 2012, Roth was honored for her work at the Hamptons International Film Festival. Here's what I learned about her illustrious decades-long career:

Never ever confuse Ann Roth’s work with fashion. From the ensembles put together for Nathan Lane in “The Birdcage,” to the flimsy house dresses worn by Sally Field in “Places in the Heart,” to the spandex, crotch and derriere defining bell-bottoms worn by Pierce Brosnan, Stellan Skarsgard and Colin Firth in “Mamma Mia!,” these are costumes. "Oh, please!" she gasps if you suggest otherwise.

She did attend: Roth, smartly clad with cardigan draped over her shoulders, became straight-man to one of the most entertaining tributes ever, featuring anecdotes galore about Roth's work, her attention to detail, her invented backstory for each character that defied the imagination of actors playing them. All this, so that the players could feel the parts, bringing award worthy performances.

As the late, beloved Mike Nichols put it, she collaborates on many aspects aside from costuming. She was responsible for Jane Fonda's Klute haircut, that then became all the rage. For Nicole Kidman as Virginia Woolf in The Hours, Roth found a key to her look at a London cobbler's shop when she was having a pair of shoes made. The craftsman said he had heard Woolf read on the radio as a boy and thought her sound had to do with her nose. Roth then suggested the infamous, transformative shnozz Kidman sported as the author of “Mrs. Dalloway.” Fonda and Kidman won the Best Actress Oscars for these roles.

But the 93rd Academy Awards eschewed the nonsense, and like the pandemic itself, made us all step up to what is necessary. While the academy honored the talent, featuring more inclusivity and diversity, the major change was women got more attention than ever. Accepting her Best Actress award, Frances McDormand noted her work is her sword. Or, as my daughter Jane observed about “Nomadland” director Chloe Zhao, she's no B.S.

From Publisher: Showbiz411



Oscars 2021: Rihanna, Paris Hilton and Amanda Seyfried attend Sunset Tower Hotel party | Daily

Amanda Seyfried was among the stars spotted attending an exclusive Academy Awards viewing party at West Hollywood's Sunset Towers on Sunday night.

The Oscar-nominated Mank star, 35, wowed as she made her arrival at the star-studded soirée in the same red Armani Privé gown she'd worn to the ceremony.

She opted for an Armani Privé dress in a fishtail silhouette with a plunging slit on the bodice, showing off a glimpse of cleavage.

Night on the town: (L-R) Amanda Seyfried, Paris Hilton, Maria Bakalova and Rihanna were among the stars spotted attending an exclusive Academy Awards viewing party at West Hollywood's Sunset Towers on Sunday night

The mother-of-two, who is married to Thomas Sadoski, paired it with a matching face mask as she arrived at the hotel in line with COVID-19 protocols.

Carrying a matching red purse and her mobile phone, the screen star gathered up her flowing gown as she exited her vehicle.

Also in attendance at the bash was Paris Hilton, 40, who went for a decidedly daring look in a shimmering semi-sheer silver gown.

Her embellished frock of choice featured a sheer peephole cutout at the cleavage, and descended into a flowing sheer skirt, which barely covered her posterior.

From Publisher: Mail Online



Oscars 2021: Paris Hilton poses with Maria Bakalova at Sunset Tower's post-Academy Awards party |

Paris Hilton looked incredible as she attended the exclusive Academy Awards viewing party at Sunset Tower's Oscar party on Sunday.

The socialite, 40, looked glamorous in a daring semi-sheer silver gown with cut-out detail as she joined stars including Rihanna and Amanda Seyfried for the bash.

She held hands with her friend, Best Supporting Actress nominee Maria Bakalova while they posed for pictures at the West Hollywood venue.

Paris stunned in the full-length shimmering dress which featured a sheer peephole cutout at the cleavage, and descended into a flowing sheer skirt, which barely covered her posterior.

The hotel heiress added extra height to her frame with a pair of black strappy heels and carried a small clutch purse as she mingled with fellow VIPs.

She was accompanied by the Borat Subsequent Moviefilm actress, 24, who wore a white mini dress with a cape and embellishment detail.

Maria paired the thigh-skimming dress with a pair of silver strappy heels and clutched a silver snakeskin purse.

From Publisher: Mail Online



Sherri Shepherd Celebrates 54th Birthday With Hollywood Themed Photo Shoot | Black America Web

Actress, comedienne, and talk show host Sherri Shepherd recently celebrated her 54th birthday (April 22) with a Hollywood-themed photoshoot and she looks absolutely stunning!

To celebrate the weight loss and her birthday, the Emmy-winning talk show host paid homage to Black women in Hollywood who've paved the way for generations to come in the entertainment industry. She tells People that the fun, classic Hollywood photoshoot was done "in the spirit of Dorothy Dandridge, Hattie McDaniel, Lena Horne, Diahann Carroll and so many others."

Sherri's makeup artist, Latrice Johnson , also shared some of the photos to her own Instagram, shouting out the multifaceted entertainer on how amazing she looked.

Sherri Shepherd Reveals Going A Year Without Sugar Has Reversed Her Type 2 Diabetes Diagnosis: "I Can't Even Believe It!"

Sherri Shepherd Celebrates 54th Birthday With Hollywood Glam Photoshoot And She Looks Fabulous! was originally published on hellobeautiful.com

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From Publisher: Black America Web



Bella Thorne Is Hot in Red at Her Engagement Party to Benjamin Mascolo

AceShowbiz - Bella Thorne made sure she turned heads while arriving at her engagement party at Los Angeles' No Vacancy. The former Disney star put her leg on display as she looked hot in a sexy red dress featuring a very high slit in one side.

For the bash which was held on Saturday evening, April 24, the 23-year-old donned the revealing dress that not only showed off a whole lotta leg, but also a ton of her cleavage. The " Midnight Sun " actress' look that night complemented her red heels and locks that she styled in a beautiful updo.

As for her fiance Benjamin Mascolo, the Italian singer/songwriter looked dapper in a black Versace tuxedo jacket featuring embroidery. He completed his look with matching shoes.

Raving about his actress fiancee, Ben shared a slew of pictures from the night. In one picture, the engaged couple was posing with a beautiful sky in the background. Another snap saw a more detailed look at Bella's custom diamond and pearl necklace bearing her fiance's name. The party also had scantily-clad dancers dancing with fire.

"My princess made this happen..," so Ben wrote in the caption. "Best engagement party ever, I'm so grateful to share these special moments with you. Also, can we talk about how insanely beautiful you are in this dress? Love you @bellathorne."

"As I've gotten older, I look so much more like my mother. I used to be really, really skinny naturally, it's taken me so long to fill out physically and my face used to be so skinny before. I used to be so much more like my father, with these very Latin high cheekbones and a very big forehead. I always felt like I looked like my father more, but now that I filled out, my face is more round and has more fat to it, so I really look like my mom. She is a redhead, like one of my sisters, I'm naturally a blonde. Now when we are together, people say we are twins," she continued.

From Publisher: AceShowbiz



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