Fans couldn't get enough of Jamie Lee Curtis ' gorgeous look at the 2021 Golden Globes over the weekend, but they might have to wait a while for another similar moment from the star.
"I'm 62 years old, and I've been wearing black leggings for a year," Curtis said on Today . "And so the truth of the matter is, I'm an actress, and I was privileged to be asked to present at the Golden Globes, and I decided to suit up and show up."
The Halloween performer also got a little cheeky when referring to her stunning physique that left social media breathless, thanks to the cleavage-baring outfit.
"And you know, once in a while, everybody needs to come out, get a little sunshine," she joked. "They are now well-fed. They are back in the stable. They don't need to come out for another five years."
The star also felt that wearing yellow provided a much-needed burst of color after what has been such a dreary past 12 months amid the pandemic.
"But there is something about wearing sunshine during this moment, when we have all been denied the freedom that sunshine and the joy of sunshine and happiness, and a little glitz and glamour," the Knives Out star continued. "And I was very happy to represent and to be able to present it, weirdly enough, to a very close friend of mine. So it was lovely."
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Grammy Awards 2021: From Skeleton Dress To Cleavage Baring Outfits, Here's Grammys RED CARPET
The Grammys has produced some of the most iconic looks in fashion history and it's the one ceremony where we can expect to see daring, wild and the weirdest looks on the red carpet and this year too some celebs stepped on the red carpet in the most unusual manner, take a look!
Miley Cyrus's younger sister Noah turn heads when she hit the red carpet wearing an ivory Schiaparelli Couture dress complete with a massive bodice.
Nominated for Best New Artist, the singer stepped out in a voluminous white Schiaparelli Couture gown, that became a hot topic of trend on social media
Phoebe Bridgers opted for a sparkling skeleton-print dress for the Grammy Awards on Sunday that obviously became the talk of the town!
She walked the red carpet in a black dress with a jewel-encrusted skeleton pattern from top to bottom.
Singer Doja Cat made jaws drop as she hit the red carpet in a feathered, plunging Roberto Cavalli x Fausto Puglisi gown that zipped all the way down past her belly button.
Singer Ingrid Andress opted for a chic ivory Armani suit, she completed the sleek look with a crystallized body chain.
Kylie Jenner Rocks See-Through Dress, Flaunts Massive Cleavage, Remains Queen of Instagram - The
But we're guessing that if we polled the entire country and asked them which celebrity first springs to mind when they think of Instagram, Kylie Jenner would rank number one.
And if you have want to know why that is, look no further than the 23-year-old billionaire's latest post:
You don't see Jeff Bezos, seductively posing on a couch, his nipples barely concealed by an impossibly thin sheer fabric.
And when was the last time Warren Buffet shot you a look that said "why don't you sit down beside me, and we'll see where the evening goes?"
Maybe Bill Gates has the ability to inspire impure thoughts in his Instagram followers, but the fact of the matter is, he's not taking advantage of his erotic powers -- and Kylie is.
That, folks, is the difference between some nerd with a bowl-cut and a 10-figure dynamo who has what it takes to be the very first President of the Entire Freakin' World!
Kylie captioned her latest post "the main character" and it's hard to argue with that self-assessment.
Instagram, the Kar-Jenner clan, the dreams of the 8 million people who liked her latest thirst trap -- Kylie is the main character in all of them.
Well, we don't want to re-open the debate over whether Kylie is really a "self-made" billionaire , but the fact is, she's had a lot of help.
Epitope antibody testing is effective in detecting immune responses to SARS-CoV-2
The authors of the study note their epitope antibody test could be helpful and more effective than conventional screening methods to identify people with the most effective plasma and antibodies for antibody therapy in the absence of a vaccine.
The researchers then searched for the exact targets of SARS-CoV-2 antibody responses. They created peptides with 89 epitopes for the S, N and membrane (M) proteins and exposed them to 15 donated plasma samples, including 14 samples from positive infection and one control.
The team found antibody responses honed in on 54 epitopes from the S and N protein. To assess the strength of an antibody response to treatment, the researchers created a ‘master epitope array’ with 16 epitopes that corresponded with strong IgG signaling.
The major 16 epitopes were screened with donated plasma from 89 COVID-19 patients and 9 controls. Findings showed that antibodies from people in the severe group in the ICU responded to more epitopes than the group with moderate infection.
The coronavirus spike protein has undergone the most mutations out of the whole coronavirus, and is the reason for the emergence of variants. To further understand this, the team created 28 mutations to the S or N proteins, which relates to the B.1.1.7 and B.1351 variant.
Results showed the mutations helped reduce or eliminate IgG binding to epitopes. Specifically, a P681H mutation changed the S-671 epitope and made it undetectable by antibodies.
The team developed a test that would measure the epitope-antibody response for coronavirus infections. When tested against donated plasma, agglutination was only found in plasma that had a positive diagnosis of COVID-19.
medRxiv publishes preliminary scientific reports that are not peer-reviewed and, therefore, should not be regarded as conclusive, guide clinical practice/health-related behavior, or treated as established information.
Jocelyn Solis-Moreira graduated with a Bachelor's in Integrative Neuroscience, where she then pursued graduate research looking at the long-term effects of adolescent binge drinking on the brain's neurochemistry in adulthood.
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Busted! What The Great and Bridgerton reveal about cleavage | Fashion | The Guardian
Take Lady Svenska, a courtier hellbent on ousting Catherine the Great. We know from her decolletage that she's a baddie long before she calls Catherine "a bitch" for refusing a biscuit. Then there's Georgina Dymov, the mistress of Catherine's husband, Peter III, whose libidinal interests are crystallised by the gold-plated pendant pointing directly towards her bosom.
"Given Georgina Dymov's character, it would frankly be odd if Georgina was more covered up," says the show's costume designer, Emma Fryer. In the language of the show, the moral worth of female nobility is weighed up in their bust. Even Catherine abandons her high necklines when she starts having sex with her lover.
If it borrows from history and plays loose with the facts, it is faithful to one thing: that contemporary fashion favoured the cantilevered look, and women were judged for the amount of cleavage they showed, depending on their age and social currency.
All too often an overt cleavage was seen as a symbol of "coquettishness or feigned youth". This is seen in the cartoons of James Gillray and George Cruikshank, who used a woman's bosom as "shorthand for impractical vanity or faux innocence". And all too often, age came into it: "Sometimes these commentaries [presented] the wearing of potentially revealing garments, particularly by older women, as poor taste," Cordner says.
For Fryer, the focus was character first, cleavage later. "It's not trying to be completely historically correct but de-robing, particularly in the background, was shown to add a sense of debauchery and anarchy," says Fryer, whose research came from the mid-Georgian portraits at the National Gallery, Givenchy and Dior, and, to an extent, reality TV.
The stratified worlds of 18th-century Russia and Regency England overlapped as much in class and imperialism as they did in fashion, at least according to these series. When it comes to cleavage, it was more of a byproduct of wearing a corset – or "stay" – rather than an explicit focus, says Cordner. Boned, and stopping around the diaphragm, they were a precursor to balcony bras. Their purpose was to support the bust and create the distinctive regency "shelf" silhouette.
These period dramas are not topical documentaries. But at their heart, are charismatic women acting as conduits through which to explore modern-day themes of sex and class . If only to prove that not that much has changed.
Socialite Nadia Fairfax wears a white mini-dress at a boat party after her wedding to Michael
Hours after tying the knot with her longtime partner financier Michael Wayne on Thursday, Nadia Fairfax changed into a sexy mini dress for the after party.
The 31-year-old socialite bride was spotted arriving in Sydney's Rose Bay to board a boat with wedding guests after the nuptials.
She was accompanied by her new husband, Michael, 33, who clasped her hand as they walked along the wharf together.
Nadia had changed out of her wedding dress and into a flirty number, which featured an open back and feathered skirt.
She rounded out her ensemble with a pair of Jimmy Choo jewelled stilettos, a small white handbag, and accessorised with silver jewellery.
Nadia wore the same hairstyle she had at her wedding, but it looked slightly dishevelled after a long day of celebrating.
Loss of furin cleavage site attenuates SARS-CoV-2 pathogenesis | Nature
a , Schematic of SARS-CoV-2 challenge, created with BioRender. b – f , Male and female mice were challenged with 10 3 PFU of wild-type (black) or ΔPRRA (blue) SARS-CoV-2, and evaluated for weight loss ( n = 12 for both groups) ( b ), and viral RNA from the lung ( c ), nasal turbinate ( d ), nasal wash ( e ) and brain ( f ). At 2 days post-infection (dpi), n = 9 mice infected with wild type, 11 mice infected with ΔPRRA; at 7 dpi, n = 11 mice for both. N gene copies in the brain were measured at 7 dpi only. LOD, limit of detection. g , h , Lung function evaluated at 7 dpi using Flexivent mechanical ventilator to assess inspiratory capacity ( g ) and pressure–volume loop ( h ). n = 10 mice infected with wild type; n = 9 mice infected with ΔPRRA. i – k , Lung histopathology at 7 dpi from mock- ( i ), wild-type- ( j ) and ΔPRRA- ( j ) infected mice. Images are representative of lung sections from three mice in all cases. Scale bar, 250 μm. l , Chemokine analysis of mouse lung homogenates at 7 dpi, from mock-infected mice (white), or mice infected with wild-type (black) or ΔPRRA (blue) SARS-CoV-2. n = 8 for all groups. Data are mean ± s.e.m. P values from two-tailed Student's t -test with unequal variance ( b ), Kruskal–Wallis Test for multiple comparisons ( c – e , g , h ), χ 2 test ( f ) or a two-tailed Mann–Whitney test between wild-type and ΔPRRA ( l ).
a , Schematic for ΔPRRA SARS-CoV-2 reporter virus expressing mNeonGreen gene in place of ORF7 . b , PRNT 50 values measured by mNeonGreen expression, with wild-type SARS-CoV-2 on the x axis and ΔPRRA SARS-CoV-2 on the y axis. c – e , Representative curves from low ( c ), intermediate ( d ) and high ( e ) neutralizing sera from patients with COVID-19. n = 3. f – h , Neutralization curves from three monoclonal antibodies (mAb 1 ( f ), mAb 2 ( g ) and mAb 3 ( h )). n = 3. i , Particle:PFU ratio determined from 40 fields, dividing into individual particles (left) and clusters (right) to determine ratios. j , Percentage of particles as individual virions (1), doubles (2) or larger clusters (>3). Data are mean ± s.e.m.
A number of approaches can prevent the emergence of the ΔPRRA mutation in SARS-CoV-2 stocks. In our studies, we found no evidence for PRRA deletion in infectious clone-derived wild-type SARS-CoV-2 through passage 2. By using low-passage stocks, the incorporation of this mutation was limited. One alternative is the use of Vero E6 cells expressing TMPRSS2, which removes the fitness advantage of the ΔPRRA mutant and will allow virus propagation without altering the full-length-to-S1/S2 processing ratio of the S protein. However, continued passage risks this or other tissue culture adaptations, and careful monitoring of stock composition is needed. Using plaque purification techniques, wild-type SARS-CoV-2 can be selected for by its smaller plaque morphology.
No statistical methods were used to predetermine sample size. The experiments were not randomized, and investigators were not blinded to allocation during experiments and outcome assessment.
For detection of viral RNA, the nasal washes and oral swabs of hamsters infected with wild-type SARS-CoV-2 or ΔPRRA SARS-CoV-2, RNA extraction, cDNA synthesis and RT–qPCR were performed as described in the preceding paragraph. For RT–qPCR, primer 1 and primer 3 were used for all hamster samples.
Supernatants of SARS-CoV-2 infected cells were centrifuged for 10 min at 3,000 g to remove large cellular debris. Nickel grids were incubated with clarified supernatants for 10 min followed by glutaraldehyde fixation and 2% uranyl acetate staining. Micrographs were taken using a JEM 14000 (JEOL USA). Several randomly selected fields were imaged to obtain unbiased particle counts.
Lung homogenates were incubated with Triton-X-100 (1% final concentration) for 1 h at room temperature to inactivate SARS-CoV-2. Homogenates then were analysed for cytokines and chemokines by Eve Technologies, using their Mouse Cytokine Array and Chemokine Array 31-Plex (MD31) platform.
Upon euthanasia, the lung was inflated with about 1.2 ml of 10% neutral buffered formalin using a 3-ml syringe and catheter inserted into the trachea. The airway, lungs and heart were removed en bloc and transferred to a conical flask containing 40 ml 10% neutral buffered formalin, in which the tissues were allowed to fix for suspension of neutral buffered formalin for ≥7 days. Tissues were embedded in paraffin, and sections were stained with haematoxylin and eosin by the Washington University Lung Morphology Core. Images were captured using the Nanozoomer (Hamamatsu) at the Alafi Neuroimaging Core at Washington University.
The recombinant wild-type and mutant SARS-CoV-2 described in this Article are available through the WRCEVA at UTMB through a material transfer agreement.
Furin cleavage SARS-CoV-2 spike deletions mostly suppressed within infected airway epithelium,
The SARS-CoV-2 spike protein has two subunits, the S1 and S2. S1 mediates viral spike-host cell angiotensin-converting enzyme 2 (ACE2) receptor binding, while S2 mediates viral entry into the host cell via membrane fusion.
The spike protein is the immunodominant antigen in human infection, as well as playing the central role in the type of host cell targeted by the virus, the pathogenicity of the virus, and its transmissibility. In nature, it occurs as a homotrimer on the surface of the viral particle.
Importantly, the SARS-CoV-2 spike is different from that of the closely related bat SARS-like coronaviruses encoding a unique polybasic furin cleavage site (FCS) at the S1/S2 interface. This is composed of four amino acids, PRRA.
The FCS gene itself is composed of the RRAR↓S sequence. Its presence in this virus, but not in other betacoronaviruses, indicates that it is crucial to the virulence of the virus by increasing the host range, the range of cell tropism, its transmissibility, and pathogenicity.
When grown in Vero cells, derived from African green monkey cells, SARS-CoV-2 rapidly produced S1/S2 interfacial mutations that included the loss of the FCS with the proximal and distal amino acids in its progeny virions. These formed a stable dominant population with repeated passaging.
The spike gene is the most variable among all SARS-CoV-2 genes, and the FCS region at the S1/S2 interface is especially prone to mutations. A variety of deletions and mutations have been identified in this region in Vero cells infected with the virus.
Especially, one mutant comprising a 30 base pair deletion was able to replicate more efficiently within Vero cells, becoming the dominant strain on passaging. Another 21-base pair deletion was also found after two or three passages. These have not been found in the clinical isolate that was originally passaged, however. This shows that these were acquired during Vero cell passages.
Clinical isolates show the presence of many deletions around the FCS. The current study aimed to understand how these arose during the growth of the virus in human airways.
They found that significant differences involved chiefly the deletions at the S1/S2 interface. The high rate of FCS deletions contributed to a much lower percentage of sgRNAs relative to all canonical sgRNAs, at only 50%, in infected HAE-ALI cells.
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